Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide analysis of pluripotency marker expression of ESKM-iPS cells


ABSTRACT: iPS cells, produced in in presence of exogeneously expressed E-cadherin and abcence of viral Oct4 were compared with conventional produced OSKM-iPS cells and murine embryonic stem cells (mESCs) or MEFs Total RNA was isolated from iPSCs and mESCs grown in presence of LIF on gelatin-coated plates for 4 days and from murine embryonic fibroblasts grown in standard medium (DMEM-Glutamax, high-glucose, 10 % FBS, 1X non-essential amino acids, penicillin/ streptomycin

ORGANISM(S): Mus musculus

SUBMITTER: Torben Redmer 

PROVIDER: E-GEOD-28594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

E-cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming.

Redmer Torben T   Diecke Sebastian S   Grigoryan Tamara T   Quiroga-Negreira Angel A   Birchmeier Walter W   Besser Daniel D  

EMBO reports 20110701 7


We report new functions of the cell-adhesion molecule E-cadherin in murine pluripotent cells. E-cadherin is highly expressed in mouse embryonic stem cells, and interference with E-cadherin causes differentiation. During cellular reprogramming of mouse fibroblasts by OCT4, SOX2, KLF4 and c-MYC, fully reprogrammed cells were exclusively observed in the E-cadherin-positive cell population and could not be obtained in the absence of E-cadherin. Moreover, reprogrammed cells could be established by vi  ...[more]

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