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Identification of Sox9-Regulated Pathways During the Secondary Transition Stage of Pancreas Development

ABSTRACT: Sox9 target genes were identified during the secondary transition stage of pancreas development by comparing gene expression in Sox9-ablated versus wild-type pancreata using microarray analysis. Sox9 was conditionally ablated during the secondary transition in the developing pancreas via recombination of a Sox9-flox allele (Kist et al., 2002) using the tamoxifen-inducible Rosa26-CreER allele (Vooijs et al., 2001). Dams were injected with 6 mg/40 g tamoxifen in corn oil at e12.5. Pancreata were manually microdissected at e15.5. Total RNA was isolated and pooled from pancreata of e15.5 Sox9fl/fl; Rosa26-CreER (mutant) versus Rosa26-CreER (wild-type) littermates for four biological replicates.

ORGANISM(S): Mus musculus

SUBMITTER: Hung Ping Shih 

PROVIDER: E-GEOD-28670 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A Notch-dependent molecular circuitry initiates pancreatic endocrine and ductal cell differentiation.

Shih Hung Ping HP   Kopp Janel L JL   Sandhu Manbir M   Dubois Claire L CL   Seymour Philip A PA   Grapin-Botton Anne A   Sander Maike M  

Development (Cambridge, England) 20120606 14

In the pancreas, Notch signaling is thought to prevent cell differentiation, thereby maintaining progenitors in an undifferentiated state. Here, we show that Notch renders progenitors competent to differentiate into ductal and endocrine cells by inducing activators of cell differentiation. Notch signaling promotes the expression of Sox9, which cell-autonomously activates the pro-endocrine gene Ngn3. However, at high Notch activity endocrine differentiation is blocked, as Notch also induces expre  ...[more]

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