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Differentially expressed genes in metallopanstimulin-1 knockdown cells

ABSTRACT: To identify the differentially expressed genes in metallopanstimulin-1 (MPS-1) knockdown gastric cancer cells compared with negative control ones, we employed the microarray profiling analysis. MPS-1 was knockdown by retroviral interfering system in human gastric adenocarcinoma originated cell SGC7901 and the transfectants named P4, while the negative control named NC. Genes with greater than 1.5-fold change and P-value ?0.05 were identified as differentially expressed genes between NC and P4 cells. Among those, apoptotic related gene (Gadd45?, cIAP2, Bcl2, CAD, Bid, etc) and adhesive related genes (integrin beta 4, ECM2, etc) were quantified by real-time PCR as well as western blotting. The two groups of negative control (NC) and MPS-1 knockdown gastric cancer cells (P4) were harvested after puromycin screening. Three independent experiments were performed for each group.

ORGANISM(S): Homo sapiens

SUBMITTER: zhongyin Yang 

PROVIDER: E-GEOD-28877 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Knockdown of metallopanstimulin-1 inhibits NF-κB signaling at different levels: the role of apoptosis induction of gastric cancer cells.

Yang Zhong-Yin ZY   Qu Ying Y   Zhang Qing Q   Wei Min M   Liu Chuan-Xu CX   Chen Xue-Hua XH   Yan Min M   Zhu Zheng-Gang ZG   Liu Bing-Ya BY   Chen Guo-Qiang GQ   Wu Ying-Li YL   Gu Qin-Long QL  

International journal of cancer 20111129 12

The ribosomal protein S27 (metallopanstimulin-1, MPS-1) has been reported to be a multifunctional protein, with increased expression in a number of cancers. We reported previously that MPS-1 was highly expressed in human gastric cancer. Knockdown of MPS-1 led to spontaneous apoptosis and repressed proliferation of human gastric cancer cells in vitro and in vivo. However, how does MPS-1 regulate these processes is unclear. Here we performed microarray and pathway analyses to investigate possible  ...[more]

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