Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Identification of GLI1 direct target genes in human pancreatic cancer cell line Panc-1


ABSTRACT: Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 3 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array GLI1 direct target genes were identified in Panc-1 cells. Gene expression profiles of Panc-1GLI1ER and Panc-1ER cells were analyzed by AGILENT human 4x44 cDNA microarray before and 3 hours after treatment of beta-estradiol (E2) at doses of 10nM.

ORGANISM(S): Homo sapiens

SUBMITTER: Shingo Inaguma 

PROVIDER: E-GEOD-28901 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2017-04-01 | GSE28902 | GEO
2013-07-18 | GSE28901 | GEO
2017-04-01 | GSE28900 | GEO
2017-04-01 | GSE28899 | GEO
2014-07-01 | E-GEOD-53237 | biostudies-arrayexpress
2014-04-14 | E-GEOD-39704 | biostudies-arrayexpress
2014-04-14 | GSE39704 | GEO
2022-03-08 | GSE197905 | GEO
2016-07-31 | E-GEOD-71272 | biostudies-arrayexpress
2011-07-29 | E-TABM-1051 | biostudies-arrayexpress