Transcriptomics

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Identification of GLI1 direct and indirect target genes in human pancreatic cancer cell line Panc-1


ABSTRACT: Panc-1GLI1ER and Panc-1ER were established from human pancreatic cancer cell line Panc-1. Panc-1GLI1ER cells express a chimeric transgene composed of an enhanced green fluorescent protein (EGFP)-tagged version of the amino-terminal half of GLI1, fused to the AF2 domain of the mouse estrogen receptor 1(Esr1) cDNA. On the other hand, Panc-1ER harbor only EGFP-tagged AF2 domain. To identify GLI1 direct target genes, total RNAs were purified from the cells before and 24 hours after the beta-estradiol (E2) treatment. Gene expression profiles were analyzed by AGILENT human 4x44 cDNA microarray. Keywords: Expression profiling by genome tiling array

ORGANISM(S): Homo sapiens

PROVIDER: GSE28902 | GEO | 2017/04/01

SECONDARY ACCESSION(S): PRJNA140661

REPOSITORIES: GEO

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