Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Study of differential gene expression in the thymus, spleen and lymphatic nodes of susceptible and resistant mice during T. cruzi infection

ABSTRACT: This is a time course study of differential gene expression in the thymus and spleen of susceptible (Balb/c) and resistant (C57BL/6) mice during experimental Trypanosoma cruzi infection. There is a special interest in the expression of galectins, glycosyltransferases, neuraminidases and chemokines in the gene expression patterns. Mice were infected with 1000 trypomastigotes of T. cruzi strain Y i.p. Organs (thymus and spleen) were removed aseptically at days 0, 7, 14, 21 and 28 post-infection. The time points have been selected as they allow the following of the acute phase of the disease with the peak of parasitemia (between 7 and 14 days), and the chronic phase (more than 21 days post infection). RNA from at least three animals from the same data point was pooled to normalize internal variability. Total RNA was isolated as per recommended protocol and shipped to the Core E site. Samples were hybridized to the GLYCOv3 array.

ORGANISM(S): Mus musculus

SUBMITTER: Steven Head

PROVIDER: E-GEOD-28921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Oral transmission of T. cruzi is probably the most frequent mechanism among animals in the wild. In this context, there is a high prevalence of human infections in regions where triatomine infection with T. cruzi is low. This led to the hypothesis that the consumption of raw or undercooked meat from animals infected with T. cruzi could be responsible for the transmission of the infection. Therefore, the general objective of this study was to demonstrate the role of meat consumption from infected animals in the oral transmission of T. cruzi infection. Groups of five female mice Balb/c were fed with muscles obtained from mice in the acute phase of infection by the clone H510 C8C3hvir of T. cruzi, and the infection of the fed mice was monitored by a parasitemia curve. Similarly, we assessed the infective capacity of T. cruzi trypomastigotes and amastigotes by infecting groups of five mice Balb/c females, which were infected orally using a nasogastric probe, and the infection was monitored by parasitemia curve. Finally, different trypomastigote and amastigote inoculums were used to determine their infective capacity. Adhesion assays of T. cruzi proteins to AGS stomach cells were performed and the adhered proteins were detected by western blotting using monoclonal or polyclonal antibodies. In all cases, 60–100% of animals were fed meat from mice infected in the acute phase or infected by means of a nasogastric probe with trypomastigotes or amastigotes. These animals developed high parasitemia, and 80% died around day 40 post-infection. The adhesion tests showed that cruzipain is a molecule of trypomastigotes and amastigotes that binds to AGS cells. LC-MS/MS also confirmed that transialidase may be involved in TCT attachment of TCT or invasion of stomach cells. It was concluded that the consumption of meat from infected animals in the acute phase of T. cruzi infection allows transmission of the infection. Similarly, trypomastigotes and amastigotes were able to infect mice when administered orally, while cruzipain was a relevant molecule in this infective process.

Dataset Information

Study of differential gene expression in the thymus, spleen and lymphatic nodes of susceptible and resistant mice during T. cruzi infection

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets