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Effects of eyestalk ablation on the gene expression levels in female black tiger shrimp broodstock (Penaeus monodon)

ABSTRACT: Comparisons of gene expression profiles between ovaries of before (day 0) and after eyestalk ablation (days 1, 4 and 7) of domesticated 14-month-old black tiger shrimp (Penaeus monodon) were made using a cDNA microarray. Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized. A cDNA microarray consisting of 5,568 features was constructed from EST libraries of P. monodon. RNA samples were extracted from the ovaries of before (day 0) and after eyestalk ablation at days 1, 4 and 7 from shrimp from the Shrimp Genetic Improvement Center, Thailand. The RNA was converted into cDNA by indirect aminoallyl-cDNA labelling method (LabelStar Array, Qiagen). aa-cDNA from day 0 samples were coupled with Cy3 dye as a reference, and those from days 1, 4 and 7 were coupled with Cy5 dye.

ORGANISM(S): Penaeus monodon

SUBMITTER: nitsara karoonuthaisiri 

PROVIDER: E-GEOD-29025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Insights into eyestalk ablation mechanism to induce ovarian maturation in the black tiger shrimp.

Uawisetwathana Umaporn U   Leelatanawit Rungnapa R   Klanchui Amornpan A   Prommoon Juthatip J   Klinbunga Sirawut S   Karoonuthaisiri Nitsara N  

PloS one 20110907 9

Eyestalk ablation is commonly practiced in crustacean to induce ovarian maturation in captivity. The molecular mechanism of the ablation has not been well understood, preventing a search for alternative measures to induce ovarian maturation in aquaculture. This is the first study to employ cDNA microarray to examine effects of eyestalk ablation at the transcriptomic level and pathway mapping analysis to identify potentially affected biological pathways in the black tiger shrimp (Penaeus monodon)  ...[more]

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