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Unknown,Transcriptomics,Genomics,Proteomics

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The transcriptome of poplar roots incubated with MiSSP7, a secreted effector protein of Laccaria bicolor

ABSTRACT: To investigate which genes are affected by MiSSP7, a secreted effector protein of Laccaria bicolor, we analyzed the transcriptomes of poplar roots incubated with MiSSP7 protein. The Populus trichocarpa custom-exon expression array (12x135K) manufactured by Roche NimbleGen Systems Limited (Madison, WI) (http://www.nimblegen.com/products/exp/index.html) contained three independent, nonidentical, 60-mer probes per gene model coding sequence. Included in the oligoarray were 43,929 annotated gene models (Populus trichocarpa genome v2 ; Phytozome 5.0), 5,859 random 60-mer control probes and labelling controls. We performed six hybridizations, three biological replicates from poplar roots inoculated with MiSSP7 peptide for one hour and three biological replicates from control roots.

ORGANISM(S): Populus tremula x Populus alba

SUBMITTER: Annegret Kohler

PROVIDER: E-GEOD-29050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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