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MicorRNA expression in klotho-deficient and wild-type mouse brain subregions

ABSTRACT: The expression profiles of miRNAs in klotho-deficient and wild-type mouse brain subregions were examined. The data indicated that little or no marked difference in the miRNA expression was detected between them. Twelve samples prevared from 4- or 5-week-old klotho-deficient and wild-type mouse brain tissues (cerebrum, cerebellum and hippocampus) were examined.

ORGANISM(S): Mus musculus

SUBMITTER: Hirohiko Hohjoh

PROVIDER: E-GEOD-29092 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Alteration of microRNA expression in the process of mouse brain growth.

Eda Akiko A Takahashi Masaki M Fukushima Tatsunobu T Hohjoh Hirohiko H

Gene 20110628 1

MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation in development, differentiation and proliferation. In this study, we investigated expression profiles of miRNAs in the process of normal mouse brain growth from embryonic day 16.5 to ~19 months old by means of DNA microarray and reverse-transcription quantitative polymerase chain reaction, and we examined whether there was any association between the expression of miRNAs and brain growth. The res ...[more]

PMID: 21718763

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Analyses of senescence-associated miRNAs in various mouse tissues

Project description:This SuperSeries is composed of the following subset Series: GSE34531: MicorRNA expression in various tissues of klotho-deficient and wild-type mice GSE34532: microRNA expression in young and elder mouse tissues Refer to individual Series

2012-11-01 | E-GEOD-34533 | biostudies-arrayexpress

microRNA expression in various tissues of klotho-deficient and wild-type mice

Project description:The expression profiles of miRNAs in klotho-deficient and wild-type mice were examined by means of GenopalM-BM-.-MICM DNA chips. The data suggested that there was a difference in the expression of miRNAs between klotho-deficient and wild-type mice. Small RNA samples prevared from 5-week-old klotho-deficient and wild-type mice were examined.

2012-11-01 | E-GEOD-34531 | biostudies-arrayexpress

microRNA expression in young and elder mouse tissues

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2012-11-01 | E-GEOD-34532 | biostudies-arrayexpress

Alteration of microRNA expression during mouse brain growing

Project description:We investigated expression profiles of miRNAs in the process of normal mouse brain growing from embryonic day 16.5 to one year old by means of DNA microarray. The results indicated that a major change in the expression of miRNAs occurred in the brain within the first week to the fourth week postnatally. The data also exhibited miRNAs which gradually increased and not decreased in their levels over the course of brain growing. Thirty-eight samples prepared from various aged mice were analyzed. Experiments were duplicated.

2011-06-08 | E-GEOD-29091 | biostudies-arrayexpress

microRNA expression in HeLa cells under a mild hyperthermia

Project description:We investigated expression profiles of miRNAs in HeLa cells that were subjected to a mild hyperthermia as well as normal temperature by means of DNA microarray. The data suggested similar expression profiles to each other. Small RNA samples prepared from HeLa cells exposed to a mild hyperthermia (40M-BM-:C) as well as normal temperature (37M-BM-:C) were examined by means of GenopalM-BM-.-MICH07 DNA chips.

2014-09-01 | E-GEOD-53745 | biostudies-arrayexpress

Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation

Project description:This SuperSeries is composed of the following subset Series: GSE10512: Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.1) GSE10513: Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.2) Keywords: SuperSeries Refer to individual Series

2008-12-21 | E-GEOD-10572 | biostudies-arrayexpress

Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.1)

Project description:In comparison with allogeneic hematopoietic stem cell transplantation using other sources, umbilical cord blood transplantation (UCBT) offers substantial clinical advantages including a lower incidence and severity of acute graft-versus-host disease (GVHD) despite the use of allogeneic UCB stem cells with more disparate HLA. However, detailed pathophysiology of acute GVHD developed after UCBT has not yet been clarified. Here, we examined the roles of inflammatory cells in the pathogenesis of acute GVHD following UCBT, by expression profiling of a total of 595 genes in each of 4 subsets (CD4+, CD8+, CD14+, and CD56+) of peripheral blood mononuclear cells (PBMCs), which were taken from 3 patients with hematologic malignancy, in whom acute GVHD had developed after UCBT. We compared expression profiles measured during acute GVHD with those measured opon discharge (recovery phase), for each subset of PBMCs to identify immuno-regulatory genes which were assumed to be linked to the pathogenesis of acute GVHD. Keywords: disease state analysis Peripheral blood mononuclear cells (PBMCs) were taken from 3 patients with hematologic malignancy, in whom acute GVHD had developed after cord blood transplantation. CD4+, CD8+, CD14+, and CD56+ cell subsets were isolated from the PBMCs of 2 patients, while only CD8+ and CD56+ cell subsets were isolated from the PBMCs of a patient. PBMCs were taken twice - during acute GVHD phase and upon discharge (recovery phase), from each patient. By using a custom-made fibrous oligonucleotide DNA microarray Genopal (Mitsubishi Rayon), we compared expression profiles of 595 unique genes measured during acute GVHD with those measured upon discharge (recovery phase), for each subset of PBMCs.

2010-05-15 | E-GEOD-10512 | biostudies-arrayexpress

Gene Expression Profiles of PBMC Subsets in Acute GVHD Following Cord Blood Transplantation (IMS v.2)

Project description:In comparison with allogeneic hematopoietic stem cell transplantation using other sources, umbilical cord blood transplantation (UCBT) offers substantial clinical advantages including a lower incidence and severity of acute graft-versus-host disease (GVHD) despite the use of allogeneic UCB stem cells with more disparate HLA. However, detailed pathophysiology of acute GVHD developed after UCBT has not yet been clarified. Here, we examined the roles of inflammatory cells in the pathogenesis of acute GVHD following UCBT, by expression profiling of a total of 615 genes in each of 4 subsets (CD4+, CD8+, CD14+, and CD56+) of peripheral blood mononuclear cells (PBMCs), which were taken from 5 patients with hematologic malignancy, in whom acute GVHD had developed after UCBT. By comparing expression profiles measured during acute GVHD with those measured upon discharge (recovery phase), for each subset of PBMCs, we identified immuno-regulatory genes which were assumed to be linked to the pathogenesis of acute GVHD. Keywords: disease state analysis CD4+, CD8+, CD14+, and CD56+ cell subsets were isolated from the peripheral blood mononuclear cells (PBMCs) of 5 patients with hematologic malignancy, in whom acute GVHD had developed after cord blood transplantation. PBMCs were taken twice â during acute GVHD phase and upon discharge (recovery phase), from each patient. By using a custom-made fibrous oligonucleotide DNA microarray Genopal (Mitsubishi Rayon), we compared expression profiles of 615 unique genes measured during acute GVHD with those measured upon discharge (recovery phase), for each subset of PBMCs.

2010-05-15 | E-GEOD-10513 | biostudies-arrayexpress

Interferon signaling related gene expression and clinical outcome of chronic hepatitis C

Project description:Chronic hepatitis C (CH) frequently developed liver cirrhosis and hepatocellular carcinoma without suitable treatment. However, peginterferon(IFN) and ribavirin therapy (combination therapy), which is present standard therapy, is imperfect effect because of several side effect. We attempt to catalog IFN related gene expression profile according to the response of combination therapy in to predict the outcome of drug-response before administration and establish the adequate treatment according to the individual clinical information. Chip1 [GPL9960-related Samples]: We quantified expression profile of 200 IFN related genes by microarray in the liver biopsy specimen from 88 CH patients without history of anti-viral therapy, 10 fatty liver patients without HCV infection were also enrolled. Chip2 [GPL9962-related Samples]: We quantified expression profile of 37 IFN related genes by microarray in the liver biopsy specimen from 88 CH patients without history of anti-viral therapy, 10 fatty liver patients without HCV infection were also enrolled.

2010-02-10 | E-GEOD-20119 | biostudies-arrayexpress

Extrarenal α-klotho expression in human tissues

Project description:α-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans.

2015-10-13 | PXD002775 | Pride

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