Dataset Information

Extrarenal α-klotho expression in human tissues

ABSTRACT: α-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebellum, Brain, Renal Medulla, Prostate Epithelium Cell Line, Epithelial Cell, Cerebral Cortex, Kidney, Artery, Renal Cortex, Parathyroid Gland, Neuron, Keratinocyte, Cell Culture, Aorta, Pancreas

SUBMITTER: Laurent Gatto

LAB HEAD: Kathryn Lilley

PROVIDER: PXD002775 | Pride | 2015-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Brain_50mug__PRM.raw	Raw
	Cerebellum_50mug_PRM.raw	Raw
	EXTRARENALALPHA-KLOTHOEXPRESSISONINHUMANTISSUES.sky	Other
	EXTRARENALALPHA-KLOTHOEXPRESSISONINHUMANTISSUES.sky.view	Other
	EXTRARENALALPHA-KLOTHOEXPRESSISONINHUMANTISSUES.skyd	Other

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Publications

α-Klotho Expression in Human Tissues.

Lim Kenneth K Groen Arnoud A Molostvov Guerman G Lu Tzongshi T Lilley Kathryn S KS Snead David D James Sean S Wilkinson Ian B IB Ting Stephen S Hsiao Li-Li LL Hiemstra Thomas F TF Zehnder Daniel D

The Journal of clinical endocrinology and metabolism 20150817 10

<h4>Context</h4>α-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of α-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy.<h4>Objective</h4>This is the first study to characterize systemwide tissue expression of transmembrane α-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based met ...[more]

PMID: 26280509

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