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Unknown,Transcriptomics,Genomics,Proteomics

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Low Zn vs high Zn in CDM

ABSTRACT: Comparison of the Streptococcus pneumoniae D39 wild-type in CDM Plus 0mM Zn and CDM plus 0.2 mM Zn Two condition design comparison of Wild-type strain including a dye swap

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Sulman Shafeeq

PROVIDER: E-GEOD-29235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional response of Streptococcus pneumoniae to Zn2+-limitation and the repressor/activator function of AdcR

Project description:Zinc (Zn2+) is an important trace metal ion that has been shown to regulate the expression of several (virulence) genes in streptococci. Previously, we analyzed the genome-wide response of S. pneumoniae to Zn2+-stress. In this work, we have performed a transcriptomic analysis to identify genes that are differentially expressed under intracellular Zn2+-limitation. This revealed a number of genes that are highly upregulated in the absence of extracellular Zn2+, amongst which the genes belonging to the regulon of the Zn2+-responsive repressor AdcR, like adcBCA, encoding a Zn2+-dependent ABC-uptake system, adcAII, encoding a Zn2+-binding lipoprotein, and also virulence genes belonging to the Pht family (phtA, phtB, phtD and phtE). Using transcriptome analysis, lacZ-reporter studies, in vitro DNA binding experiments, and in silico operator predictions, we show that AdcR directly represses the promoters of adcRCBA, adcAII-phtD, phtA, phtB and phtE in the presence of Zn2+. AdcR can also function as an activator, since in the presence of Zn2+ it directly induces expression of adh that encodes a Zn2+-containing alcohol dehydrogenase. In conclusion, the genome-wide transcriptional response of S. pneumoniae to Zn2+-limitation was established, which is mainly mediated via direct regulation by the Zn2+-dependent regulator AdcR. Two condition design comparison of Wild-type strain including a dye swap Refer to individual Series. This SuperSeries is composed of the following subset Series: GSE29234: adcR mutant vs wild type D39 GSE29235: Low Zn vs high Zn in CDM

2011-05-13 | E-GEOD-29236 | biostudies-arrayexpress

adcR mutant vs wild type D39

Project description:Comparison of the Streptococcus pneumoniae D39 adcR mutant vs D39 wild type in CDM plus 0.2mM Zn One condition design comparison of two strains including a dye swap

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ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae

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Project description:Comparison of the Streptococcus pneumoniae D39 strain with 0mM compared to 0.05 mM in CDM Two condition design comparision of one strains including a dye swap

2011-07-10 | E-GEOD-30413 | biostudies-arrayexpress

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ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Glucose at MID-log growth phase

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