Thiouracil Pulse-chase, Tachyzoites and Bradyzoites mRNA stability data
Ontology highlight
ABSTRACT:
ORGANISM(S): Toxoplasma gondii
SUBMITTER: Michael Cleary
PROVIDER: E-GEOD-2947 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Biosynthetic labeling of RNA with uracil phosphoribosyltransferase allows cell-specific microarray analysis of mRNA synthesis and decay.
Cleary Michael D MD Meiering Christopher D CD Jan Eric E Guymon Rebecca R Boothroyd John C JC
Nature biotechnology 20050130 2
Standard microarrays measure mRNA abundance, not mRNA synthesis, and therefore cannot identify the mechanisms that regulate gene expression. We have developed a method to overcome this limitation by using the salvage enzyme uracil phosphoribosyltransferase (UPRT) from the protozoan Toxoplasma gondii. T. gondii UPRT has been well characterized because of its application in monitoring parasite growth: mammals lack this enzyme activity and thus only the parasite incorporates (3)H-uracil into its nu ...[more]
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