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Genome-wide analysis of atrial left-right differences in two different mouse strains

ABSTRACT: Two wild-type mouse strains (Swiss Agouti and MF1) were investigated regarding the mRNA expression patterns of their left and right atria at 12 months of age. Eight samples of each strain, four with left atrium and four with right atrium examined.

ORGANISM(S): Mus musculus

SUBMITTER: Peter Kahr

PROVIDER: E-GEOD-29500 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic analysis of gene expression differences between left and right atria in different mouse strains and in human atrial tissue.

Kahr Peter C PC Piccini Ilaria I Fabritz Larissa L Greber Boris B Schöler Hans H Scheld Hans H HH Hoffmeier Andreas A Brown Nigel A NA Kirchhof Paulus P

PloS one 20111019 10

<h4>Background</h4>Normal development of the atria requires left-right differentiation during embryonic development. Reduced expression of Pitx2c (paired-like homeodomain transcription factor 2, isoform c), a key regulator of left-right asymmetry, has recently been linked to atrial fibrillation. We therefore systematically studied the molecular composition of left and right atrial tissue in adult murine and human atria.<h4>Methods</h4>We compared left and right atrial gene expression in healthy, ...[more]

PMID: 22039477

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Genome-wide analysis of atrial left-right differences in two different mouse strains

Project description:Two wild-type mouse strains (Swiss Agouti and MF1) were investigated regarding the mRNA expression patterns of their left and right atria at 12 months of age.

2011-12-31 | GSE29500 | GEO

RNA-sequencing of left and right atrial

Project description:The right and left atria have different susceptibilities towards developing arrhythmias, with left atrial arrhythmias more commonly observed. To study potential underlying causes of this difference between the two upper chambers of the heart, four human left-right atrial pairs were subjected to whole-genome expression analyses via next generation sequencing of small RNAs, including microRNAs (miRNAs), and polyA enriched mRNAs. Using a paired sample design, significant differences in gene expression were found between the left and right atria in both the poly-A and small RNA fractions. Hsa-miR-143 was the most highly expressed miRNA in the atria as quantified by RNA-seq. Gene expression differences established during development are retained into adulthood including that of PITX2 and BMP10. In addition ten novel non-coding RNAs were found to be differentially expressed between the left and right atrias .

2011-09-10 | GSE31999 | GEO

Valvular heart disease and atrial fibrillation regulate microRNA expression profiles in left and right atria differently

Project description:Background: Chronic atrial fibrillation (AF) is a complication associated with the dilated atria of patients with valvular heart disease and contributes to worsened pathology. Methods and Results: Using microarray technology, we examined microRNA (miR) expression profiles in right and left atrial appendage tissue from valvular heart disease (VHD) patients. Right atrial appendage from patients undergoing coronary artery bypass grafting (CABG) and left atrial (LA) appendage from healthy hearts not used for transplant were used as controls. VHD induced different changes in miR expression in LA compared with right atria (RA). Fifty-two (52) miRs were altered by VHD in LA, compared with 5 in RA tissue. There was no detectable effect of chronic AF on miR expression in LA tissue, but miR expression in RA was strongly influenced by AF, with 47 miRs showing differential expression. LA volume correlated with miR expression changes in both LA and RA, but the affected miRs were different for the two atrial groups. Conclusions: VHD and AF influence miR expression patterns in LA and RA, but these are affected differently by disease progression and by the development of AF. These findings provide new insights into the progression of VHD. RA tissue is not a useful surrogate for LA in studies of mitral valve disease. 34 arrays from either the left or right atrium from patients with Valvular Heart Disease (VHD), patients undergoing coronary artery bypass grafting (CABG), or healthy controls. Arrays in this series were generated on V2 and V3 Agilent microRNA arrays and analysed in combination.

2011-12-21 | E-GEOD-28954 | biostudies-arrayexpress

Atrial fibrillation is associated with altered left-to-right atria gene expression ratio: implications for arrhythmogenesis and thrombogenesis

Project description:Background: Atrial fibrillation (AF) causes atrial remodeling, and the left atrium (LA) is the favored substrate for maintaining AF. However, it remains unclear if AF remodels both atria differently and contributes to LA arrhythmogenesis and thrombogenesis. Results: AF was associated with differential LA-to-RA gene expression related to specific ion channels and pathways as well as upregulation of thrombogenesis-related genes in the LA appendage. Targeting the molecular mechanisms underlying the LA-to-RA difference and AF-related remodeling in the LA appendage may help provide new therapeutic options in treating AF and preventing thromboembolism in AF. Paired left atrial and right atrial specimens were obtained from 13 patients with persistent AF receiving valvular surgery. The Paired specimens were sent for microarray comparison. Selected results were validated by quantitative real time-PCR (q-PCR) and Western blotting. Ultrastructural changes in the atria were evaluated by immunohistochemistry.

2016-08-09 | E-GEOD-79768 | biostudies-arrayexpress

Differentially Expressed Genes for Atrial Fibrillation Identified using RNA Sequencing from Paired Human Left and Right Atrial Appendages.

Project description:Next Generation RNA Sequencing was carried out on human paired left and right atrial appendages from patients with and without Atrial Fibrillation. EdgeR software was used to show a total of 247 genes were found to have significant differential expression between left and right atria.

2019-03-13 | GSE128188 | GEO

Left-right transcriptomic differences in adult male mouse heart atria

Project description:Left and right heart atria of adult male mice were profiled to determine the differences in gene expression, control, coordination and signaling fabrics Two-sides (L= left, R = right) gene expression profiling experiment in adult mouse male (M) atria (A). 4 biological replicates: MAL1-4, MAR1-4. This experiment used our standard multiple yellow strategy in which Cy3 and Cy5 labeled biological replicates are cohybridized with a two-color array and each channel is processed independently.

2013-03-20 | E-GEOD-45339 | biostudies-arrayexpress

Gene expression profiling of right atrium and left ventricle

Project description:A comparison of human cardiac gene expression profile in paired samples of right atrium and left ventricle extracted in vivo<br><br>

2012-01-30 | E-MEXP-3396 | biostudies-arrayexpress

Differential transcriptional responses to cardiomyocyte-specific Notch activation in right versus left atria of murine cardiac tissue

Project description:We report the mRNA sequencing of right and left atria from an adult inducible, cardiomyocyte-specific Notch gain-of-function mouse model (iNICD). Using the tetracycline-on system, we activated Notch by feeding doxycycline chow for 3 weeks to mice that were at least 8 weeks old. We asked what transcriptional changes occur in right versus left atrial cardiomyocytes in response to the same stimulus (Notch signaling). mRNA sequencing on separated right and left atria revealed that there are more differentially dysregulated transcripts (1,011) than similarly regulated transcripts (447) in the right and left atria, which is a simiar paradigm as what occurs in human atrial cardiomyocytes of patients with atrial fibrillation.

2020-07-01 | GSE138253 | GEO

Left-right transcriptomic differences in adult male mouse heart atria

Project description:Left and right heart atria of adult male mice were profiled to determine the differences in gene expression, control, coordination and signaling fabrics

2013-03-20 | GSE45339 | GEO

Valvular heart disease and atrial fibrillation regulate microRNA expression profiles in left and right atria differently

2011-12-21 | GSE28954 | GEO