Entamoeba heat shock experiments
Ontology highlight
ABSTRACT:
ORGANISM(S): Entamoeba histolytica
SUBMITTER: Ryan Macfarlane
PROVIDER: E-GEOD-2978 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transcriptional profiling of Entamoeba histolytica trophozoites.
MacFarlane Ryan C RC Shah Preetam H PH Singh Upinder U
International journal for parasitology 20050401 5
We have developed an Entamoeba histolytica genomic DNA microarray and used it to develop a transcriptional profile of 1,971 E. histolytica (HM-1:IMSS) genes. The arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by Northern blot analysis or RT-PCR respectively). Genes expressed at high levels ( approximately 2% of total) included actin (8.m00351), and ribosomal genes (20.m00312). Moderately expressed ...[more]
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