Unknown,Transcriptomics,Genomics,Proteomics

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Identification of Notch targets in human endothelial cells


ABSTRACT: Human umbilical vein endothelial cells (HUVECs) were transduced with either MIY-N1IC (Notch1 intracellular domain) or MIY vector control. The cells were sorted for YFP, and RNA was extracted using Trizol (Invitrogen) and analyzed by the Affymetrix Human Genome U133 Plus 2.0 Array. Results were analyzed using the GCRMA algorithm to identify genes with a minimum of 2-fold induction or reduction. This global gene expression study was used to identify Notch targets in the endothelium. Expression profiling of primary HUVECs transduced with N1IC. HUVEC primary cells were isolated, transduced with N1IC or vector (MIY) control in three biological replicates, and evaluated on Affymetrix Human Genome U133 Plus 2.0 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Alex Chang 

PROVIDER: E-GEOD-29850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Notch initiates the endothelial-to-mesenchymal transition in the atrioventricular canal through autocrine activation of soluble guanylyl cyclase.

Chang Alex C Y AC   Fu YangXin Y   Garside Victoria C VC   Niessen Kyle K   Chang Linda L   Fuller Megan M   Setiadi Audi A   Smrz Justin J   Kyle Alastair A   Minchinton Andrew A   Marra Marco M   Hoodless Pamela A PA   Karsan Aly A  

Developmental cell 20110801 2


The heart is the most common site of congenital defects, and valvuloseptal defects are the most common of the cardiac anomalies seen in the newborn. The process of endothelial-to-mesenchymal transition (EndMT) in the cardiac cushions is a required step during early valve development, and Notch signaling is required for this process. Here we show that Notch activation induces the transcription of both subunits of the soluble guanylyl cyclase (sGC) heterodimer, GUCY1A3 and GUCY1B3, which form the  ...[more]

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