Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from 35S::VvCBF4-overexpressing grapevines


ABSTRACT: Overexpression of a grapevine C-repeat binding factor (CBF) gene, VvCBF4 in cv. Freedom was found to improve freezing survival in non-cold-acclimated vines. To understand the mechanistic basis of VvCBF4 transgene action, a transgenic line ('9-12') was genotyped using microarray-based mRNA expression profiling. Young grapevines (cv. 'Freedom') were grown in soil for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare vines transformed with the VvCBF4 transcription factor, expressed under the 35S CaMV promoter (line '9-12') vs. an empty vector transformant (line '8-6'). We harvested the whole aerial tissues of four biological replicates, each.

ORGANISM(S): Vitis vinifera

SUBMITTER: Richard Tillett 

PROVIDER: E-GEOD-29948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Vitis vinifera C-repeat binding protein 4 (VvCBF4) transcriptional factor enhances freezing tolerance in wine grape.

Tillett Richard L RL   Wheatley Matthew D MD   Tattersall Elizabeth A R EA   Schlauch Karen A KA   Cramer Grant R GR   Cushman John C JC  

Plant biotechnology journal 20110913 1


Chilling and freezing can reduce significantly vine survival and fruit set in Vitis vinifera wine grape. To overcome such production losses, a recently identified grapevine C-repeat binding factor (CBF) gene, VvCBF4, was overexpressed in grape vine cv. 'Freedom' and found to improve freezing survival and reduced freezing-induced electrolyte leakage by up to 2 °C in non-cold-acclimated vines. In addition, overexpression of this transgene caused a reduced growth phenotype similar to that observed  ...[more]

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