Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from control and high sugar-fed third instar Drosophila larvae


ABSTRACT: Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. To gain insight into the mechanisms underlying this response, we profiled gene expression in chronically high sugar fed, wandering (post-prandial) third instar wild type larvae (L3). These data were compared to control-fed larvae as well as those (mid-L3) actively feeding for twelve hours on both diets. We used microarrays to detail the response of Drosophila larvae to high sugar-induced insulin resistance. Male Canton-S third instar larvae were fed control (0.15M) or high (1M) sucrose and selected for RNA extraction and hybridization on Affymetrix microarrays. Wandering L3 were selected as those in the top half of the vial with partial blue guts to confirm that they had stopped eating the (blue) food. Mid-L3 were selected as L2, aged overnight until early L3, then transferred to fresh control or high sucrose food for 12 more hours before selection.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Thomas Baranski 

PROVIDER: E-GEOD-30020 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A high-sugar diet produces obesity and insulin resistance in wild-type Drosophila.

Musselman Laura Palanker LP   Fink Jill L JL   Narzinski Kirk K   Ramachandran Prasanna Venkatesh PV   Hathiramani Sumitha Sukumar SS   Cagan Ross L RL   Baranski Thomas J TJ  

Disease models & mechanisms 20110630 6


Insulin-resistant, 'type 2' diabetes (T2D) results from a complex interplay between genes and environment. In particular, both caloric excess and obesity are strongly associated with T2D across many genetic backgrounds. To gain insights into how dietary excess affects insulin resistance, we studied the simple model organism Drosophila melanogaster. Larvae reared on a high-sugar diet were hyperglycemic, insulin resistant and accumulated fat--hallmarks of T2D--compared with those reared on control  ...[more]

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