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Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of the fat body from bigmax mutant and wildtype third instar Drosophila melanogaster larvae

ABSTRACT: To identify genes differentially expressed in the fatbody of Drosphila melanogaster bigmax mutants, a loss-of-function allele was generated by P-element mobilization. Mutant and wildtype first instar larvae were raised on two different sources of food, control and high-sugar media. When the animals reached the wandering third instar stage, animals were sacrificed and their fat bodies dissected. Total RNA was extracted, labeled fluorescently and hybridized competitively to Agilent's 4x44K Drosophila Gene Expression Microarrays. On each array, three different samples were analyzed: 1. wildtype animals raised on control food, 2. wildtype animals raised on high-sugar food and 3. bigmax mutant animals raised on high-sugar food.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Thomas Sandmann

PROVIDER: E-MTAB-699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mondo/ChREBP-Mlx-regulated transcriptional network is essential for dietary sugar tolerance in Drosophila.

Havula Essi E Teesalu Mari M Hyötyläinen Tuulia T Seppälä Heini H Hasygar Kiran K Auvinen Petri P Orešič Matej M Sandmann Thomas T Hietakangas Ville V

PLoS genetics 20130404 4

Sugars are important nutrients for many animals, but are also proposed to contribute to overnutrition-derived metabolic diseases in humans. Understanding the genetic factors governing dietary sugar tolerance therefore has profound biological and medical significance. Paralogous Mondo transcription factors ChREBP and MondoA, with their common binding partner Mlx, are key sensors of intracellular glucose flux in mammals. Here we report analysis of the in vivo function of Drosophila melanogaster Ml ...[more]

PMID: 23593032

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