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Analysis of DNA methylation in a three-generation family reveals widespread genetic influence on epigenetic regulation

ABSTRACT: To assess variation and inheritance of genome-wide patterns of DNA methylation simultaneously in humans, we applied reduced representation bisulfite sequencing (RRBS) to somatic DNA from six members of a three-generation family. Reduced representation bisulfite sequencing was applied to genomic DNA from leukocytes of 6 family members and two unrelated individuals.

ORGANISM(S): Homo sapiens

SUBMITTER: Jason Gertz

PROVIDER: E-GEOD-30253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Analysis of DNA methylation in a three-generation family reveals widespread genetic influence on epigenetic regulation.

Gertz Jason J Varley Katherine E KE Reddy Timothy E TE Bowling Kevin M KM Pauli Florencia F Parker Stephanie L SL Kucera Katerina S KS Willard Huntington F HF Myers Richard M RM

PLoS genetics 20110811 8

The methylation of cytosines in CpG dinucleotides is essential for cellular differentiation and the progression of many cancers, and it plays an important role in gametic imprinting. To assess variation and inheritance of genome-wide patterns of DNA methylation simultaneously in humans, we applied reduced representation bisulfite sequencing (RRBS) to somatic DNA from six members of a three-generation family. We observed that 8.1% of heterozygous SNPs are associated with differential methylation ...[more]

PMID: 21852959

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