Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

40 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MRNA expression profiling in mouse bronchoalveolar stem cells (BASC)

ABSTRACT: We performed miRNA and mRNA profiling in BASC cells and c-Myc depleted BASC cells. We built potential miRNA-mRNA interaction networks specific to c-Myc regulation in BASCs Replicated profilling for both BASC cells and c-Myc depleted BASC cells.

ORGANISM(S): Mus musculus

SUBMITTER: Jie Dong

PROVIDER: E-GEOD-30323 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

c-Myc regulates self-renewal in bronchoalveolar stem cells.

Dong Jie J Sutor Shari S Jiang Guoqian G Cao Yajun Y Asmann Yan W YW Wigle Dennis A DA

PloS one 20110817 8

<h4>Background</h4>Bronchoalveolar stem cells (BASCs) located in the bronchoalveolar duct junction are thought to regenerate both bronchiolar and alveolar epithelium during homeostatic turnover and in response to injury. The mechanisms directing self-renewal in BASCs are poorly understood.<h4>Methods</h4>BASCs (Sca-1(+), CD34(+), CD31(-) and, CD45(-)) were isolated from adult mouse lung using FACS, and their capacity for self-renewal and differentiation were demonstrated by immunostaining. A tra ...[more]

PMID: 21858211

Similar Datasets

MicroRNA expression profile in mouse bronchoalveolar stem cells (BASC)

Project description:We performed miRNA and mRNA profiling in BASC cells and c-Myc depleted BASC cells. We built potential miRNA-mRNA interaction networks specific to c-Myc regulation in BASCs Replicated profilling for both BASC cells and c-Myc depleted BASC cells.

2012-01-26 | E-GEOD-30435 | biostudies-arrayexpress

mRNA and miRNA expression profiling in mouse bronchoalveolar stem cells (BASC)

Project description:This SuperSeries is composed of the following subset Series: GSE30323: mRNA expression profiling in mouse bronchoalveolar stem cells (BASC) GSE30435: MicroRNA expression profile in mouse bronchoalveolar stem cells (BASC) Refer to individual Series

2012-01-26 | E-GEOD-30437 | biostudies-arrayexpress

iCLIP Analysis of WT, phospho-defective, and phospho-mimicking mutants of mouse Nucleolin in iKras PDAC cells

Project description:This experiment aimed to identify Nucleolin (Ncl) binding sites on a transcriptome-wide scale, by performing iCLIP in mouse iKras PDAC cells (Ying et al., 2012) transiently transfected with a mammalian expression construct encoding a wild-type (WT) myc-Ncl, a phospho-defective mutant in which S28, S34, S40, and S41 residues were mutated to A, or a phospho-mimicking mutant with these residues mutated to D. Cells transfected with an empty vector (myc-pRK5-DEST) were used as controls in the experiment.

2023-03-01 | E-MTAB-12481 | biostudies-arrayexpress

Murine alveolar macrophage cell line MH-S was transduced with lentivirus expressing GFP alone or GFP plus murine MFSD7C tagged with Myc and FLAG epitopes.

Project description:We used immunoprecipitation (IP) and mass spectrometry (MS) to identify proteins that interact with MFSD7C. Because an anti-MFSD7C monoclonal antibody is not available, we used MFSD7C tagged with both Myc and FLAG epitopes in a MFSD7C-expressing murine alveolar macrophage cell line MH-S (Supplementary Fig. 2a-c and see Methods for details). A total of 58 proteins were identified excluding MFSD7C and common contaminants of IP experiments such as keratin, actin, and ribosomal proteins (Table 1). Twenty-six of the 58 proteins are annotated as mitochondrial proteins and 4 (three Fc receptors and one integrin) are known to reside in the cytoplasmic membrane. The 58 proteins were classified into 10 different functional categories based on gene ontology.

2020-08-20 | PXD021016 | Pride

A sumoylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis

Project description:Myc is an oncogenic transcription factor frequently dysregulated in human cancer. To identify pathways supporting the Myc oncogenic program, we employed a genome-wide RNAi screen for Myc-synthetic-lethal (MySL) genes and uncovered a role for the SUMO-activating-enzyme (SAE1/2). Loss of SAE1/2 enzymatic activity drives synthetic lethality with Myc. Mechanistically, SAE2 inhibition switches a transcriptional subprogram of Myc from activated to repressed. A subset of these SUMOylation-dependent Myc-switchers (SMS genes) governs mitotic spindle function and is required to support the Myc oncogenic program. comparison of 4 treatments: normal HMEC, High Myc in HMEC, SUMO depleted in HMEC, High Myc+Sumo Depleted in HMEC

2011-12-08 | E-GEOD-34055 | biostudies-arrayexpress

MicroRNA expression profile in mouse bronchoalveolar stem cells (BASC)

Project description:We performed miRNA and mRNA profiling in BASC cells and c-Myc depleted BASC cells. We built potential miRNA-mRNA interaction networks specific to c-Myc regulation in BASCs

2012-01-26 | GSE30435 | GEO

mRNA expression profiling in mouse bronchoalveolar stem cells (BASC)

Project description:We performed miRNA and mRNA profiling in BASC cells and c-Myc depleted BASC cells. We built potential miRNA-mRNA interaction networks specific to c-Myc regulation in BASCs

2012-01-26 | GSE30323 | GEO

Atrial Myxomas Arise From Multipotent Myxoid Cardiac Stem Cells

Project description:Myxomas, the most common primary tumor of the heart, usually develop in the atria and consist of a myxoid matrix composed of an acid-mucopolysaccharide-rich stroma with polygonal stromal cells scattered throughout the matrix. These benign tumors, despite their rarity, are a research focus because of their clinical presentation and uncertain histogenesis. The objective of this study was to assess whether adult cardiac stem/progenitor cells (CSCs) give rise to myxoma stromal cells and secrete the typical myxoid matrix. 23 collected tumors showed the typical histological features of cardiac atrial myxoma with polygonal cells positive for the myxoma tumor-cell marker, calretinin, dispersed in an abundant myxoid matrix. We detected myxoma cells positive for c-kit (c-kitpos) but very rare Isl-1 positive cells. Most of these c-kitpos cells were lineage-committed CD45pos/CD31pos cells. However, c-kitpos /CD45neg/CD31neg cardiac myxoma cells expressed stemness and cardiac progenitor cell transcription factors. Some (<10%) of these c-kitpos/ CD45neg/CD31neg/ myxoma cells expressed also calretinin, representing myxoma stromal precursor cells. c-kitpos/CD45neg/CD31neg cardiac myxoma cells secrete in vitro chondroitin-6-sulfate and hyaluronic acid, composing the gelatinous matrix of cardiac myxoma in vivo. In vitro, c-kitpos/CD45neg/CD31neg myxoma cells have stem cell properties being clonogenic, self-renewing and sphere forming. On the other hand, they exhibited an abortive cardiac differentiation potential with significant changes in their mRNA and microRNA transcriptome compared to normal c-kitpos/CD45neg /CD31neg CSCs. Importantly, myxoma-derived CSCs seed human atrial myxoma in xenograft’s experiments in NOD/SCID mice. Thus, un-committed c-kitpos/CD45neg /CD31neg cells fulfill the criteria of myxoma stem cells in atrial myxoma. Myxomas appear to be the first CSC-related human cardiac disease.

2020-05-02 | E-MTAB-8642 | biostudies-arrayexpress

Atrial Myxomas Arise From Multipotent Myxoid Cardiac Stem Cells

2020-04-01 | E-MTAB-8701 | biostudies-arrayexpress

Defining the molecular properties of the repairing epithelium during DSS induced colitis

Project description:In order to understand the molecular changes associated with the establishment of the repairing epithelium following dextran sulfate sodium (DSS) induced injury of the colon we have isolated Epcam+Sca1+ expressing epithelial cells from repairing tissue and Epcam+ homeostatic intestine. Cells isolated by flow cytometry were subjected to RNA extraction and analysed by expression analysis

2017-11-30 | E-MTAB-5249 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data