Dataset Information

Murine alveolar macrophage cell line MH-S was transduced with lentivirus expressing GFP alone or GFP plus murine MFSD7C tagged with Myc and FLAG epitopes.

ABSTRACT: We used immunoprecipitation (IP) and mass spectrometry (MS) to identify proteins that interact with MFSD7C. Because an anti-MFSD7C monoclonal antibody is not available, we used MFSD7C tagged with both Myc and FLAG epitopes in a MFSD7C-expressing murine alveolar macrophage cell line MH-S (Supplementary Fig. 2a-c and see Methods for details). A total of 58 proteins were identified excluding MFSD7C and common contaminants of IP experiments such as keratin, actin, and ribosomal proteins (Table 1). Twenty-six of the 58 proteins are annotated as mitochondrial proteins and 4 (three Fc receptors and one integrin) are known to reside in the cytoplasmic membrane. The 58 proteins were classified into 10 different functional categories based on gene ontology.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Macrophage

DISEASE(S): Proliferative Vasculopathy And Hydranencephaly-hydrocephaly Syndrome

SUBMITTER: Yingzhong Li

LAB HEAD: Jianzhu Chen

PROVIDER: PXD021016 | Pride | 2020-08-20

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	140828_64_YL_7C.raw	Raw
	140828_64_YL_GFP.raw	Raw
	2016-06-14Lietalproteomicdata.xlsx	Xlsx

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Publications

MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme.

Li Yingzhong Y Ivica Nikola A NA Dong Ting T Papageorgiou Dimitrios P DP He Yanpu Y Brown Douglas R DR Kleyman Marianna M Hu Guangan G Chen Walter W WW Sullivan Lucas B LB Del Rosario Amanda A Hammond Paula T PT Vander Heiden Matthew G MG Chen Jianzhu J

Nature communications 20200924 1

ATP synthesis and thermogenesis are two critical outputs of mitochondrial respiration. How these outputs are regulated to balance the cellular requirement for energy and heat is largely unknown. Here we show that major facilitator superfamily domain containing 7C (MFSD7C) uncouples mitochondrial respiration to switch ATP synthesis to thermogenesis in response to heme. When heme levels are low, MSFD7C promotes ATP synthesis by interacting with components of the electron transport chain (ETC) comp ...[more]

PMID: 32973183

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