Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional response of Streptococcus pneumoniae to copper

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE30413: High low copper GSE30414: copY Mutant compared to D39 wild-type Refer to individual Series

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Sulman Shafeeq

PROVIDER: E-GEOD-30415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The cop operon is required for copper homeostasis and contributes to virulence in Streptococcus pneumoniae.

Shafeeq Sulman S Yesilkaya Hasan H Kloosterman Tomas G TG Narayanan Geetha G Wandel Michal M Andrew Peter W PW Kuipers Oscar P OP Morrissey Julie A JA

Molecular microbiology 20110719 5

High levels of copper are toxic and therefore bacteria must limit free intracellular levels to prevent cellular damage. In this study, we show that a number of pneumococcal genes are differentially regulated by copper, including an operon encoding a CopY regulator, a protein of unknown function (CupA) and a P1-type ATPase, CopA, which is conserved in all sequenced Streptococcus pneumoniae strains. Transcriptional analysis demonstrated that the cop operon is induced by copper in vitro, repressed ...[more]

PMID: 21736642

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Project description:Streptococcus pneumoniae is a strictly fermentative human pathogen that relies on carbohydrate metabolism to generate energy for growth. The nasopharynx colonised by the bacterium is poor in free sugars, but mucosa lining glycans can provide a source of sugar. In blood and inflamed tissues glucose is the prevailing sugar. As a result during progression from colonisation to disease S. pneumoniae has to cope with a pronounced shift in carbohydrate nature and availability. Thus, we set out to assess the pneumococcal response to sugars found in glycans and the influence of glucose (Glc) on this response at the transcriptional, physiological and metabolic levels. Galactose (Gal), N-acetylglucosamine (GlcNAc) and mannose (Man) affected the expression of 8 to 14% of the genes covering cellular functions including central carbon metabolism and virulence. The pattern of end-products as monitored by in vivo 13C-NMR is in good agreement with the fermentation profiles during growth, while the pools of phosphorylated metabolites are consistent with the type of fermentation observed (homolactic vs. mixed) and regulation at the metabolic level. Furthermore, the accumulation of -Gal6P and Man6P indicate metabolic bottlenecks in the metabolism of Gal and Man, respectively. Glc added to cells actively metabolizing other sugar(s)was readily consumed and elicited a metabolic shift towards a homolactic profile. The transcriptional response to Glc was large (over 5% of the genome). In central carbon metabolism (most represented category), Glc exerted mostly negative regulation. The smallest response was observed on a sugar mix, suggesting that exposure to varied sugars improves the fitness of S. pneumoniae. The expression of classical virulence factors was negatively controlled by Glc in a sugar-dependent manner. Overall, our results shed new light on the link between carbohydrate metabolism, adaptation to host niches and virulence. Two conditions with the same strain (D39), including a dye swap

Dataset Information

Transcriptional response of Streptococcus pneumoniae to copper

Publications

The cop operon is required for copper homeostasis and contributes to virulence in Streptococcus pneumoniae.

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