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Transcription profiling of human hair follicles from men and women

ABSTRACT: Investigation of Gene Expression Profiling in Unstaged Head Hair Follicles Plucked from Men and Women Experiment Overall Design: Total RNA was extracted from 1-3 unstaged follicles plucked from the scalp of each of 36 adult human volunteers. The average quantifiable yield of RNA per follicle was 112.5 ng. Ten samples were selected, based on ribosomal ratio, relative integrity number and total yield, for expression profiling. By preamplifying the extracted RNA (starting with as little as 30 ng), sufficient labeled RNA was generated to conduct Afymetrix-based gene expression analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: David Dix

PROVIDER: E-GEOD-3058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in head hair follicles plucked from men and women.

Kim Sung Jae SJ Dix David J DJ Thompson Kary E KE Murrell Rachel N RN Schmid Judith E JE Gallagher Jane E JE Rockett John C JC

Annals of clinical and laboratory science 20060101 2

Characterizing gene expression in hair follicles can help to elucidate the hair growth cycle by delineating the genes and pathways involved in follicular growth and degeneration. The objectives of this study were to determine whether intact RNA could be extracted from a small number of plucked, unstaged hair follicles in sufficient quantity to conduct gene expression profiling, and to conduct global gene expression profiling. To this end, RNA was extracted from 1 to 3 unstaged follicles plucked ...[more]

PMID: 16682506

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