Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Analysis of Gene expression of human Th17 and Th1 clones


ABSTRACT: Human T cell clones were derived according to standard protocols using different mix of cytokines, once assessed for their functional profile were studied for gene expression. The purpose of this study is to analyze human Th1 and Th17 clones with and without stimulation with PMA and Ionomycin to measure differences in gene expression that can distinguish the two lineages. Human T cell clones were derived according to standard protocols using different mix of cytokines, once assessed for their functional profile were studied for gene expression

ORGANISM(S): Homo sapiens

SUBMITTER: Raffaele De Palma 

PROVIDER: E-GEOD-30664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


The reason why CD4(+) T helper 17 (Th17) cells, despite their well-known pathogenic role in chronic inflammatory disorders, are very rare in the inflammatory sites remains unclear. We demonstrate that human Th17 cells exhibit low ability to proliferate and to produce the T cell growth factor interleukin-2 (IL-2), in response to combined CD3 and CD28 stimulation. This was due to the upregulated expression of IL-4-induced gene 1 (IL4I1) mRNA, a secreted L-phenylalanine oxidase, which associated wi  ...[more]

Similar Datasets

2012-02-27 | GSE30664 | GEO
2013-10-31 | E-GEOD-44774 | biostudies-arrayexpress
2013-10-31 | E-GEOD-44776 | biostudies-arrayexpress
2013-10-31 | E-GEOD-44773 | biostudies-arrayexpress
2010-11-13 | E-GEOD-25278 | biostudies-arrayexpress
2010-07-01 | E-GEOD-22642 | biostudies-arrayexpress
2008-05-30 | E-GEOD-11553 | biostudies-arrayexpress
2016-04-06 | E-GEOD-79939 | biostudies-arrayexpress
2016-04-06 | E-GEOD-79940 | biostudies-arrayexpress
2016-04-06 | E-GEOD-79938 | biostudies-arrayexpress