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Isolation and in vitro expansion of human colonic stem cells [Expression profile]

ABSTRACT: Using the surface marker EPHB2, we have FACS-purified and profiled stem cell-enriched cell fractions from normal human mucosa, crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal human colon epithelial stem cells We FACS purified human colonic crypt cells according to their EPHB2 surface abundance. We used Affymetrix chips to hybridize 3 samples from EPHB2-high, 3 samples from EPHB2-medium, 3 samples from EPHB2-low, and 2 samples from EPHB2-negative cells

ORGANISM(S): Homo sapiens

SUBMITTER: Eduard Batlle

PROVIDER: E-GEOD-31255 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Isolation and in vitro expansion of human colonic stem cells.

Jung Peter P Sato Toshiro T Merlos-Suárez Anna A Barriga Francisco M FM Iglesias Mar M Rossell David D Auer Herbert H Gallardo Mercedes M Blasco Maria A MA Sancho Elena E Clevers Hans H Batlle Eduard E

Nature medicine 20110904 10

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-h ...[more]

PMID: 21892181

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2011-03-14 | E-GEOD-27605 | biostudies-arrayexpress

Isolation and in vitro expansion of human colonic stem cells

Project description:This SuperSeries is composed of the following subset Series: GSE31255: Isolation and in vitro expansion of human colonic stem cells [Expression profile] GSE31256: Isolation and in vitro expansion of human colonic stem cells [CGH] Refer to individual Series

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