Transcription profiling of mouse colon tissue taken 3-12 hours after injection of ephrinB2-Fc
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ABSTRACT: To gain a global view of the signaling pathways engaged by EphB receptors in the intestinal epithelium, we analyzed the transcriptional alteraltions after acute inhibition of EphB signaling in vivo. An intravenous injection of ephrin-B2-Fc decreases EphB2 tyrosine phosphorylation and results in a reduction in cell proliferation to a similar extent as in EphB2:EphB3 double null mutant mice. Moreover, cells bevome mislocalized along the crypt-villus axis, as in the EphB2;EphB3 --/-- mice, although this is not apparent until several days after injection. By analysing the transcriptome within the first day after blocking EphB signaling, it is possible to study the effect of blocked EphB signaling without the potential secondary effects caused by distorted cell positioning. Since ephrin-B2-Fc binds exclusively to EphB expressing cells inthe crypts in the intestine, it is possible to assess transcriptional alterations in these cells in response to EphB antagonist in whole preparations of colon.
ORGANISM(S): Mus musculus
SUBMITTER: Maria Genander
PROVIDER: E-TABM-753 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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