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SmRNA sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator

ABSTRACT: Deep sequencing of smRNA from queen and virgin queen of two Ants: Camponotus floridanus and Harpegnathos saltator Total RNA were isolated from virgin queens and queens by pulverizing the entire animal after removal of gut and poison glands in liquid N2 and then dissolving in TRIzol. Gel-purified 18-30 nts RNAs were used for sequencing.

ORGANISM(S): Harpegnathos saltator

SUBMITTER: hong cao 

PROVIDER: E-GEOD-31344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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<h4>Background</h4>Ant societies comprise individuals belonging to different castes characterized by specialized morphologies and behaviors. Because ant embryos can follow different developmental trajectories, epigenetic mechanisms must play a role in caste determination. Ants have a full set of DNA methyltransferases and their genomes contain methylcytosine. To determine the relationship between DNA methylation and phenotypic plasticity in ants, we obtained and compared the genome-wide methylom  ...[more]

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