Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiling of in vitro matured bovine oocytes cultivated with or without cumulus cells.


ABSTRACT: Cumulus cells, surrounding the oocyte, play a key role in the acquisition of oocyte competence to be fertilized and to sustain early embryo development. Cumulus cells contribute to oocyte development by metabolizing energy substrates such as glutathione that may protect the oocyte from oxidative stress damages. The aim of our study was to compare transcriptomics profiles of cumulus enclosed (CEO) and cumulus denuded (CDO) oocytes after in vitro maturation. Global transcriptional profiling was performed using cumulus enclosed and cumulus denuded oocytes after in vitro maturation. Matured oocytes were obtained after 22h of maturation with (CEO) or without (CDO) cumulus cells and four replicates of 25 oocytes were collected for RNA extraction. Gene expression analysis was performed by comparing CDO versus CEO oocytes that represents a total of 8 slides using a dye swap hybridisation protocol.

ORGANISM(S): Bos taurus

SUBMITTER: Svetlana UZBEKOVA 

PROVIDER: E-GEOD-31361 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2011-08-08 | E-GEOD-31261 | biostudies-arrayexpress
2011-08-17 | GSE31361 | GEO
2011-12-10 | E-GEOD-19327 | biostudies-arrayexpress
2015-04-10 | E-GEOD-44792 | biostudies-arrayexpress
2019-04-02 | PXD012141 | Pride
2012-10-14 | E-GEOD-36604 | biostudies-arrayexpress
2008-06-15 | E-GEOD-7225 | biostudies-arrayexpress
2011-04-25 | E-GEOD-23449 | biostudies-arrayexpress
2017-04-10 | PXD004892 | Pride
2017-03-05 | E-MTAB-5400 | biostudies-arrayexpress