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High resolution methylome map of rat reveals role of intragenic DNA methylation in identifying coding region and alternative splice site.

ABSTRACT: We report the application of next generation sequencing technology for high-throughput profiling of cytosine methylation in adult rat liver tissue. To investigate the role of intragenic methylation, we generated the first high resolution methylation map of rat liver tissue and compared it with the proteome of the same rat tissue.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Shantanu Gupta

PROVIDER: E-GEOD-31571 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High resolution methylome map of rat indicates role of intragenic DNA methylation in identification of coding region.

Sati Satish S Tanwar Vinay Singh VS Kumar K Anand KA Patowary Ashok A Jain Vaibhav V Ghosh Sourav S Ahmad Shadab S Singh Meghna M Reddy S Umakar SU Chandak Giriraj Ratan GR Raghunath Manchala M Sivasubbu Sridhar S Chakraborty Kausik K Scaria Vinod V Sengupta Shantanu S

PloS one 20120215 2

DNA methylation is crucial for gene regulation and maintenance of genomic stability. Rat has been a key model system in understanding mammalian systemic physiology, however detailed rat methylome remains uncharacterized till date. Here, we present the first high resolution methylome of rat liver generated using Methylated DNA immunoprecipitation and high throughput sequencing (MeDIP-Seq) approach. We observed that within the DNA/RNA repeat elements, simple repeats harbor the highest degree of me ...[more]

PMID: 22355382

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