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Intragenic DNA inversions expand bacterial coding capacity

ABSTRACT: Bacteria can generate heterogeneity through phase variation, including enzyme-mediated inversion of specific intergenic regions of genomic DNA. We developed a computation tool that identifies invertible elements using long-read datasets, PhaVa. We identified over 300 ‘intragenic invertons,’ a new class of invertible elements entirely within genes, in both bacteria and archaea. In the gut commensal Bacteroides thetaiotaomicron, we experimentally characterized an intragenic inverton in the thiamine biosynthesis protein thiC. We used mass spectrometry in data-independent acquisition mode to identify and quantify B. theta proteins, including thiC inverton expression at the protein level.

INSTRUMENT(S): timsTOF Ultra

ORGANISM(S): Bacteroides Thetaiotaomicron (strain Atcc 29148 / Dsm 2079 / Nctc 10582 / E50 / Vpi-5482)

SUBMITTER: Krystal Lum

LAB HEAD: Ileana M. Cristea

PROVIDER: PXD054577 | Pride | 2024-08-06

REPOSITORIES: Pride

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			Action	DRS
	20240507_BTheta_DIA_LockedForward_Slot2-40_1_3103.d.zip	Other
	20240507_BTheta_DIA_LockedReverse_Slot2-45_1_3111.d.zip	Other
	20240507_BTheta_DIA_UnlockedForward_Slot2-37_1_3100.d.zip	Other
	20240507_BTheta_DIA_UnlockedReverse_Slot2-38_1_3101.d.zip	Other
	20240507_BTheta_DIA_WT_Slot2-39_1_3102.d.zip	Other

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