Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Orphan Nuclear Receptors ERRα/γ are competence factors for somatic cell reprogramming


ABSTRACT: We report the expression profiles of the nuclear receptor family of transcription factors, known regulators of metabolism, during iPSC generation. Unique but overlapping expression patterns were found in iPSCs derived from adipose derived stem cells (ADSCs) and embryonic fibroblasts (human and mouse) that correlate with developmental transitions in the cell. Total RNA was obtained from human embryonic fibroblast IMR90 cells (IMR90s) untreated, transduced with GFP or with the four reprogramming factors (Oct4, Sox2, Klf4, c-Myc; OSKM) at day 5 of reprogramming.

ORGANISM(S): Homo sapiens

SUBMITTER: Ruth Yu 

PROVIDER: E-GEOD-31704 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Cell metabolism is adaptive to extrinsic demands; however, the intrinsic metabolic demands that drive the induced pluripotent stem cell (iPSC) program remain unclear. Although glycolysis increases throughout the reprogramming process, we show that the estrogen-related nuclear receptors (ERRα and ERRγ) and their partnered co-factors PGC-1α and PGC-1β are transiently induced at an early stage, resulting in a burst of oxidative phosphorylation (OXPHOS) activity. Upregulation of ERRα or ERRγ is requ  ...[more]

Similar Datasets

2015-05-27 | GSE31704 | GEO
2013-07-22 | E-GEOD-48201 | biostudies-arrayexpress
2018-02-09 | GSE94928 | GEO
2014-12-15 | E-GEOD-53330 | biostudies-arrayexpress
2014-12-15 | E-GEOD-53331 | biostudies-arrayexpress
2014-12-15 | E-GEOD-53329 | biostudies-arrayexpress
2018-02-09 | GSE94980 | GEO
2018-02-09 | GSE94979 | GEO
2020-03-27 | GSE139376 | GEO
2019-03-14 | GSE118677 | GEO