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Expression analysis of G9C8 clone and NOD.G9C8 tg CTLs


ABSTRACT: Diabetogenic CD8+ G9C8 clone cells and the T cells from a transgenic mouse bearing the same TCR as the clone, displayed differences in their ability to induce disease in vivo.Microarray analysis was done to identify the molecular basis for such differences between the two sets of CD8 T cells. Microarray analysis was done to identify the molecular basis for such differences between the two sets of CD8 T cells 3 vials of frozen G9C8 clone cells and spleen cells from 2 NOD.G9C8 transgenic mice were similarly peptide stimulated for 3 days and rested in IL2 for 2 days in order to obtain uniformly activated cells for analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Alexander Chervonsky 

PROVIDER: E-GEOD-31726 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cytotoxic mechanisms employed by mouse T cells to destroy pancreatic β-cells.

Varanasi Vineeth V   Avanesyan Lia L   Schumann Desiree M DM   Chervonsky Alexander V AV  

Diabetes 20120706 11


Several cytotoxic mechanisms have been attributed to T cells participating in β-cell death in type 1 diabetes. However, sensitivity of β-cells to these mechanisms in vitro and in vivo is likely to be different. Moreover, CD4⁺ and CD8⁺ T cells may use distinct mechanisms to cause β-cell demise that possibly involve activation of third-party cytotoxic cells. We used the transfer of genetically modified diabetogenic T cells into normal, mutant, and bone marrow chimeric recipients to test the contri  ...[more]

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