Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mtb-mediated host transcriptional reponse is strain-specific

ABSTRACT: CDC1551 induced more vigorous immune response in murine bone marrow derived macrophage (BMM). In contrast, in HN878-infected cells, host transcriptional response was delayed but lasted longer. HN878 induced more genes involved in host lipid metabolism than CDC1551 did. Murine bone marrow derived macrophages were infected with M. tuberculosis CDC1551 or HN878 up to 24 h. Total RNA was processed for microarray and global gene expression was read.

ORGANISM(S): Mus musculus

SUBMITTER: Mi-Sun Koo

PROVIDER: E-GEOD-31734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Mtb-mediated host transcriptional reponse is strain-specific

Project description:CDC1551 induced more vigorous immune response in murine bone marrow derived macrophage (BMM). In contrast, in HN878-infected cells, host transcriptional response was delayed but lasted longer. HN878 induced more genes involved in host lipid metabolism than CDC1551 did.

2011-08-30 | GSE31734 | GEO

Early innate immunity determines outcome of Mycobacterium tuberculosis pulmonary infection in rabbits

Project description:In our rabbit model of pulmonary tuberculosis, infection with Mtb HN878, a hyper-virulent W-Beijing strain, results in progressive cavitary disease. However, infection of rabbit lungs with Mtb CDC1551, a hyper-immunogenic strain is effectively controlled overtime, establishing latent Mtb infection. Using these two Mtb strains, we tested the hypothesis that the initial host response in the lungs within hours of infection determines later outcome. The microarray experiments was performed to identify gene expression changes in the Mtb-HN878 or CDC1551- infected rabbit lungs at 3 hours post infection, compared to uninfected naïve rabbit lungs. New Zealand White rabbits were infected with Mtb HN878 or CDC1551 at ~3.5log10. At 3 hours post infection, lung tissue from Mtb-infected and uninfected rabbits were isolated and used for total RNA extraction. Total rabbit lung RNA was used for microarray analysis to determine infection induced changes in host gene expression.

2013-08-17 | E-GEOD-49947 | biostudies-arrayexpress

Early innate immunity determines outcome of Mycobacterium tuberculosis pulmonary infection in rabbits

2013-08-17 | GSE49947 | GEO

Effect of PDE4 inhibition on host gene expression of Mtb-infected mouse lungs.

Project description:Global gene expressions of Mtb-infected mouse lungs were compared between with and without PDE4 inhibitor treatment. A lot of host genes are differentially expressed 21d and 28d post-Mtb infection. PDE4 inhibitor, however, downregulate 10% of genes among those and genes differentially regulated by PDE4 inhibitor are mainly involved immune response. Total RNA was isolated from Mtb-infected mouse lungs with or without CC-3052 (25 mg/kg/day) using Trizol reagent and gene profile was analyed using Affymetrix mouse ST 1.0

2010-11-13 | E-GEOD-25313 | biostudies-arrayexpress

Infection of bone marrow derived macrophages with M. tuberculosis CDC1551

Project description:I infected 8 flasks of resting murine bone marrow derived macrophages with M. tuberculosis CDC1551. Individual samples of intracellular Mtb were collected at 2hr, and days 2,4,6,8,10,12, and 14 post-infection using the GTC (guanidine thiocyanate) method as previously published. A 2hr "no macrophage" control was used as a common denominator in the two-color hybridizations. I have two independent biological replicates of this experiment. Groups of assays that are related as part of a time series. Time: culture time post-infection

2012-07-12 | GSE35362 | GEO

p16INK4a-deficiency promotes macrophages from classical to alternative activation

Project description:murine p16ink4a deficient (p16ko) and control (p16wt) bone marrow cells were either differentiated with normal LCM-supplemented differentiation medium to obtain bone marrow derived macrophages (BMDM) or supplemented with Interleukin 4 during differeniation to obtain M2 polarized p16wt and p16ko BMDM.

2011-07-01 | E-MEXP-3216 | biostudies-arrayexpress

Infection of bone marrow derived macrophages with M. tuberculosis CDC1551

2012-07-11 | E-GEOD-35362 | biostudies-arrayexpress

Mycobacterium tuberculosis H37Rv

Project description:Infection of bone marrow derived macrophages with M. tuberculosis CDC1551

| PRJNA152631 | ENA

Bulk RNA seq of sorted CD11c cells from lungs of uninfected and Mtb infected wild type mice.

Project description:To understand the difference between CD11c population from the uninfected and MTB HN878 infected mice at transcriptome level.

2024-07-05 | GSE249963 | GEO

iPSC-derived macrophages effectively treat pulmonary alveolar proteinosis in Csf2rb-deficient mice

Project description:The key aim of this experiment is to characterize the iPSC-Mφ population before and after pulmonary transplantation. For this purpose the following cell populations were compared: (i) transplanted iPSC-Mφ, (ii) Mφ obtained by in vitro differentiation of murine lineage-negative bone marrow cells (BM-Mφ), (iii) non-differentiated CD45.1 iPSC, (iv) murine alveolar Mφ (AMφ) isolated from the BALF of healthy control mice, and (v) iPSC-Mφ recovered from the transplanted animals two months after (PMT-Mφ).

2018-07-10 | E-MTAB-6442 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data