Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Wheat Yr39 and yr39 (Alpowa) genotypes treated with P.s. tritici PST-78

ABSTRACT: Comparison of 2 P. s. tritici-inoculated and mock-inoculated genotypes that differ for the Yr39 high-temperature adult-plant resistance phenotype over a time course (12, 24, 48h) ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tristan Coram. The equivalent experiment is TA11 at PLEXdb.] Yr39 and yr39 isolines 12, 24, and 48h after inoculation with P. s. tritici

ORGANISM(S): Triticum aestivum

SUBMITTER: Tristan Coram

PROVIDER: E-GEOD-31756 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome analysis of high-temperature adult-plant resistance conditioned by Yr39 during the wheat-Puccinia striiformis f. sp. tritici interaction.

Coram Tristan E TE Settles Matthew L ML Chen Xianming X

Molecular plant pathology 20080701 4

Stripe rust [caused by Puccinia striiformis Westend. f. sp. tritici Eriks. (Pst)] is a destructive disease of wheat (Triticum aestivum L.) worldwide. High-temperature adult-plant (HTAP) resistance to stripe rust is race non-specific, inherited quantitatively and durable. Previously, we identified and mapped the single Yr39 HTAP stripe rust resistance gene in the spring wheat cultivar Alpowa, which was identified on chromosome 7BL and accounted for 64.2% of the variation in resistance. To identif ...[more]

PMID: 18705862

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Project description:Transcriptional changes were monitored in the wheat cultivar Avocet*6/Yr1 following inoculation with avirulent and virulent wheat yellow rust (Puccinia striiformis f.sp. tritici) isolates using the Affymetrix wheat genome array GeneChip®. Seedlings of Avocet*6/Yr1 were grown to growth stage 12-13 (Zadoks et al., 1974) before inoculating with either the P.s. f.sp. tritici isolate 169E136 (Yr1-virulent) or 232E137 (Yr1-avirulent) or with talc for the Mock inoculation (Boyd and Minchin 2001). Plants were grown under a 16/8 hour photoperiod cycle, supplemented with sodium lighting (240 ?mol m-2 s-1) at day/night temperatures of 20°C/15°C and a relative humidity of 60%, both before and after inoculation, in a spore-free, containment level 2 greenhouse. Leaf samples from six seedlings were collected 6, 12, 24, 48 and 72 hpi for RNA extraction and transcriptomics analysis from three independent biological experiments. Leaf tissue was ground under liquid nitrogen and total RNA extracted using the TRIzol reagent method, following the manufacturer’s instructions (Invitrogen, Carlsbad, CA). RNA from each time point (16 ?g) were pooled to obtain 80 ?g of total RNA for each treatment (mock inoculated control; avirulent isolate inoculated; virulent isolate inoculated). Pooled RNA samples were further purified using the Qiagen RNeasy mini-kit according to the manufacturer instructions (Qiagen) and RNA integrity was confirmed using the Agilent 2100 Bioanalyzer (Agilent). Affymetrix GeneChip processing, including RNA quality control, microarray hybridisation and data acquisition was performed through contract research services by the John Innes Genome Laboratory, John Innes Centre, Norwich, U.K. A total of nine hybridisations were performed. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Osman Bozkurt. The equivalent experiment is TA25 at PLEXdb.] pathogen isolates: Mock inoculated (Control)(3-replications); pathogen isolates: Yr1-avirulent wheat yellow rust isolate 232E137(3-replications); pathogen isolates: Yr1-virulent wheat yellow rust isolate 169E136(3-replications)

Dataset Information

Wheat Yr39 and yr39 (Alpowa) genotypes treated with P.s. tritici PST-78

Publications

Transcriptome analysis of high-temperature adult-plant resistance conditioned by Yr39 during the wheat-Puccinia striiformis f. sp. tritici interaction.

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