Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Detection of Transposon Mutants That Cannot Survive in Macrophages and Mice.


ABSTRACT: An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. We have adapted a microarray-based transposon tracking strategy for use with a Salmonella enterica serovar Typhimurium cDNA microarray in order to identify genes important for survival and replication in RAW 264.7 mouse macrophage-like cells or in the spleens of BALB/cJ mice. A 50,000-CFU transposon library of S. enterica serovar Typhimurium strain SL1344 was serially passaged in cultured macrophages or intraperitoneally inoculated into BALB/cJ mice. The bacterial genomic DNA was isolated and processed for analysis on the microarray. The novel application of this approach to identify mutants unable to survive in cultured cells resulted in the identification of components of Salmonella pathogenicity island 2 (SPI2), which is known to be critical for intracellular survival and replication. Computed

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Kaman Chan 

PROVIDER: E-GEOD-3179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Microarray-based detection of Salmonella enterica serovar Typhimurium transposon mutants that cannot survive in macrophages and mice.

Chan Kaman K   Kim Charles C CC   Falkow Stanley S  

Infection and immunity 20050901 9


DNA microarrays provide an opportunity to combine the principles of signature-tagged mutagenesis (STM) with microarray technology to identify potentially important bacterial virulence genes. The scope of DNA microarrays allows for less laborious screening on a much larger scale than possible by STM alone. We have adapted a microarray-based transposon tracking strategy for use with a Salmonella enterica serovar Typhimurium cDNA microarray in order to identify genes important for survival and repl  ...[more]

Similar Datasets

2010-06-24 | E-GEOD-15361 | biostudies-arrayexpress
2006-01-06 | E-GEOD-3988 | biostudies-arrayexpress
2006-01-27 | E-GEOD-4111 | biostudies-arrayexpress
2010-06-24 | E-GEOD-15297 | biostudies-arrayexpress
2008-02-07 | E-GEOD-7947 | biostudies-arrayexpress
2010-05-12 | E-GEOD-18151 | biostudies-arrayexpress
2007-09-13 | E-GEOD-9027 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16103 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16107 | biostudies-arrayexpress
2005-08-11 | E-GEOD-3107 | biostudies-arrayexpress