Unknown,Transcriptomics,Genomics,Proteomics

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Yeast Membrane Bound Polysome


ABSTRACT: Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. mRNA species bound to membrane-associated polysomes were separated from other mRNAs by sedimentation equilibrium or sedimentation velocity. The distribution of individual transcripts in the 'membrane-bound' and 'cytosolic' fractions was quantitated for thousands of genes by hybridization to DNA microarrays. Transcripts known to encode secreted or membrane proteins were enriched in the membrane-bound fractions, whereas those known to encode cytoplasmic proteins were enriched in the fractions containing mRNAs associated with free and cytoplasmic ribosomes. On this basis, we identified over 275 human genes and 285 yeast genes that are likely to encode previously unrecognized secreted or membrane proteins. Computed

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Max Diehn 

PROVIDER: E-GEOD-3180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Large-scale identification of secreted and membrane-associated gene products using DNA microarrays.

Diehn M M   Eisen M B MB   Botstein D D   Brown P O PO  

Nature genetics 20000501 1


Membrane-associated and secreted proteins are an important class of proteins and include receptors, transporters, adhesion molecules, hormones and cytokines. Although algorithms have been developed to recognize potential amino-terminal membrane-targeting signals or transmembrane domains in protein sequences, their accuracy is limited and they require knowledge of the entire coding sequence, including the N terminus, which is not currently available for most of the genes in most organisms, includ  ...[more]

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