Unknown,Transcriptomics,Genomics,Proteomics

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Partially degraded RNA from bladder washing is a suitable sample for studying gene expression profiles of bladder cancer

ABSTRACT: The aims of this study were to determine the impact of different levels of RNA degradation as well as to ascertain if the gene expression profiles obtained from bladder washing correlates to that obtained from the related bladder tumor. We obtained tissue tumor and bladder washing tumor samples from the same individual. We degraded each intact RNA to 3 different degradation states with dye swap (2x4x2 arrays). All tumor RNAs were compared against a pool of 4 healthy control samples with intact RNA (named as C0).

ORGANISM(S): Homo sapiens

SUBMITTER: Moises Albareda

PROVIDER: E-GEOD-3192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Partially degraded RNA from bladder washing is a suitable sample for studying gene expression profiles in bladder cancer.

Mengual Lourdes L Burset Moisès M Ars Elisabet E Ribal María José MJ Lozano Juan José JJ Minana Belén B Sumoy Lauro L Alcaraz Antonio A

European urology 20060615 6

<h4>Objectives</h4>To determine the impact of different levels of RNA degradation on gene expression measurements and to ascertain if the gene expression profile obtained from bladder washing (BW) correlates to that obtained from the related bladder tumour (BT).<h4>Methods</h4>BT and BW RNAs from the same patient were heat shocked to obtain three RNA degradation states, which were compared with intact RNAs from healthy bladders by using complementary DNA (cDNA) microarrays. All samples were ampl ...[more]

PMID: 16815626

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Dataset Information

Partially degraded RNA from bladder washing is a suitable sample for studying gene expression profiles of bladder cancer

Publications

Partially degraded RNA from bladder washing is a suitable sample for studying gene expression profiles in bladder cancer.

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