MATT mapping of Transposon insertions sites of strain G27 GPS-cat library
Ontology highlight
ABSTRACT:
ORGANISM(S): Helicobacter pylori
SUBMITTER: Nina Salama
PROVIDER: E-GEOD-3197 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Global transposon mutagenesis and essential gene analysis of Helicobacter pylori.
Salama Nina R NR Shepherd Benjamin B Falkow Stanley S
Journal of bacteriology 20041201 23
We have constructed a genome-saturating mutant library of the human gastric pathogen Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the position of 5,363 transposon mutants in our library. While we generally found insertions well distributed throughout the genome, 344 genes had no detectable transposon insertions, and this list is predicted to be highly enriched for essential genes. Comparison to the essential gene set of other bacteria revealed a surpris ...[more]
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