Unknown,Transcriptomics,Genomics,Proteomics

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Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae)

ABSTRACT: We generated 38-bp Illumina reads from single messenger RNA libraries from three diverse developmental stages of the two-spotted spider mite to capture small RNA diversity across development. Adult, nymphal+larvae and embryonic stages were separated using sieves of various pore sizes, and mites of various developmental stages were carefully selected for small RNA library preparation. Samples were a mix of males and females to capture male and female patterns of small RNA composition and were reared on beans (Phaseolus vulgaris cv California Red Kidney). Small RNA reads were used for miRNA prediction, piRNA discovery, and for quantitation of small RNA-generating loci (i.e. expression across development). Examination of small RNA from spider mites of adult, embryonic and pooled larval/nymphal developmental stages.

ORGANISM(S): Tetranychus urticae

SUBMITTER: Richard Clark

PROVIDER: E-GEOD-32005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The genome of Tetranychus urticae reveals herbivorous pest adaptations.

Grbić Miodrag M Van Leeuwen Thomas T Clark Richard M RM Rombauts Stephane S Rouzé Pierre P Grbić Vojislava V Osborne Edward J EJ Dermauw Wannes W Ngoc Phuong Cao Thi PC Ortego Félix F Hernández-Crespo Pedro P Diaz Isabel I Martinez Manuel M Navajas Maria M Sucena Élio É Magalhães Sara S Nagy Lisa L Pace Ryan M RM Djuranović Sergej S Smagghe Guy G Iga Masatoshi M Christiaens Olivier O Veenstra Jan A JA Ewer John J Villalobos Rodrigo Mancilla RM Hutter Jeffrey L JL Hudson Stephen D SD Velez Marisela M Yi Soojin V SV Zeng Jia J Pires-daSilva Andre A Roch Fernando F Cazaux Marc M Navarro Marie M Zhurov Vladimir V Acevedo Gustavo G Bjelica Anica A Fawcett Jeffrey A JA Bonnet Eric E Martens Cindy C Baele Guy G Wissler Lothar L Sanchez-Rodriguez Aminael A Tirry Luc L Blais Catherine C Demeestere Kristof K Henz Stefan R SR Gregory T Ryan TR Mathieu Johannes J Verdon Lou L Farinelli Laurent L Schmutz Jeremy J Lindquist Erika E Feyereisen René R Van de Peer Yves Y

Nature 20111123 7374

The spider mite Tetranychus urticae is a cosmopolitan agricultural pest with an extensive host plant range and an extreme record of pesticide resistance. Here we present the completely sequenced and annotated spider mite genome, representing the first complete chelicerate genome. At 90 megabases T. urticae has the smallest sequenced arthropod genome. Compared with other arthropods, the spider mite genome shows unique changes in the hormonal environment and organization of the Hox complex, and al ...[more]

PMID: 22113690

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Project description:While pathogen-induced immunity is comparatively well characterized, far less is known about plant defense responses to arthropod herbivores. To date, most molecular-genetic studies of plant-arthropod interactions have focused on insects. However, plant-feeding (phytophagous) mites are also pests of diverse plants, and mites induce different patterns of damage to plant tissues than do well-studied insects (e.g., Lepidopteran larvae or aphids). The two-spotted spider mite, Tetranychus urticae, is among the most significant mite pests in agriculture. T. urticae is an extreme generalist that has been documented on a staggering number of plant hosts (more than 1,100), and is renowned for the rapid evolution of pesticide resistance. To understand reciprocal interactions between T. urticae and a plant host at the molecular level, we examined mite herbivory using Arabidopsis thaliana. Despite differences in feeding guilds, we found that transcriptional responses of A. thaliana to mite herbivory generally resembled those observed for insect herbivores. In particular, defense to mites was mediated by jasmonic acid (JA) biosynthesis and signaling. Further, indole glucosinolates dramatically increased mite mortality and development times. Variation in both basal and activated levels of these defense pathways might also explain differences in mite damage and feeding success between A. thaliana accessions. On the herbivore side, a diverse set of genes associated with detoxification of xenobiotics was induced upon exposure to increasing levels of in planta indole glucosinolates. Our findings provide molecular insights into the nature of, and response to, herbivory for a representative of a major class of arthropod herbivores. We used microarray to assess global gene expresion in Arabidopsis thaliana upon Tetranychus urticae attack in two A. thaliana accessions: Bla-2, resistant to spider mite herbivory and Kon, susceptible to spider mite herbivory. 3 week old Arabidopsis thaliana plants were subjected to Tetranychus urticae attack through application of 10 mites for various periods of time (timecourse scenario) or hundreds of mites for 1 hour (feeding site scenario).

Dataset Information

Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae)

Publications

The genome of Tetranychus urticae reveals herbivorous pest adaptations.

Similar Datasets

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