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Spt6 Levels are Modulated by PAAF1 and 26S Proteasome to Regulate the HIV-1 LTR and a Subset of Cellular Genes involved in Cancer.

ABSTRACT: Effect of PAAF1 and Spt6 knock-down on gene expression. Total RNAs were extracted from HeLa-LTR-Luc cells in which PAAF1 or Spt6 had been knock down by a specific siRNA or HeLa-LTR-Luc cells that had been transfected with a control siRNA (“si Neg”). Differentially expressed genes in PAAF1 knock-down cells were identified by microarray. Two biological replicates (A / B) in 3 experimental conditions (si-Neg, si-PAAF1, si-Spt6) were analyzed in a single channel experiment.

ORGANISM(S): Homo sapiens

SUBMITTER: Kevin Lebrigand 

PROVIDER: E-GEOD-32033 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR.

Nakamura Mirai M   Basavarajaiah Poornima P   Rousset Emilie E   Beraud Cyprien C   Latreille Daniel D   Henaoui Imène-Sarah IS   Lassot Irina I   Mari Bernard B   Kiernan Rosemary R  

Retrovirology 20120208

<h4>Background</h4>Tat-mediated activation of the HIV-1 promoter depends upon a proteasome-associated factor, PAAF1, which dissociates 26S proteasome to produce 19S RP that is essential for transcriptional elongation. The effect of PAAF1 on proteasome activity could also potentially shield certain factors from proteolysis, which may be implicated in the transcriptional co-activator activity of PAAF1 towards the LTR.<h4>Results</h4>Here, we show that Spt6 is targeted by proteasome in the absence  ...[more]

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