Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

49 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Isw1 and Chd1 maintain chromatin organization during transcription

ABSTRACT: ChIP-chip assays to determine the localization of 3xFLAG tagged Ioc4, Ioc3 & Isw1 in wildtype and set2 yeast. Two color experiment. ChIP/Input. Biological replicates=3.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Michaela Smolle

PROVIDER: E-GEOD-32042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Localization of Isw1-3xFLAG in wild-type yeast.

Project description:ChIP-chip assays to determine the localisation of 3xFLAG tagged Isw1 in wild-type yeast. Two color experiment. ChIP/Input. Biological replicates=3. Other data used in this study are provided in GEO Series GSE31015 and GSE31833.

2011-09-06 | E-GEOD-31301 | biostudies-arrayexpress

Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange

Project description:This SuperSeries is composed of the following subset Series: GSE32042: Isw1 and Chd1 maintain chromatin organization during transcription GSE32043: Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains GSE32044: Histone H3 K36me3 in wildtype, isw1 and chd1 yeast strains GSE32045: Isw1 and Chd1 maintain chromatin organization during transcription [AcH4 data] GSE36405: Isw1 and Chd1 maintain chromatin organization during transcription [K56ac data] GSE37158: Isw1 and Chd1 maintain chromatin organization during transcription [isw1 chd1 mutant expression] Refer to individual Series

2012-07-24 | E-GEOD-32071 | biostudies-arrayexpress

Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains

Project description:Histone exchange in wildtype, isw1, chd1, isw1 chd1 and ioc4 yeast strains. Two color experiment. Deletion mutant vs. WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-32043 | biostudies-arrayexpress

Isw1 and Chd1 maintain chromatin organization during transcription [K56ac data]

Project description:ChIP-chip assays to determine the occupancy of acetylated histone H3 K56 in wildtype, isw1, chd1, isw1 chd1 and ISW1[K227R] yeast. Two color experiment. ChIP/Input. K56ac data normalized to histone H3 and plotted as mutant over wildtype. Biological replicates=3. Please note that H3 data for YMS123-125 & YMS127 is part of the AcH4 file.

2012-07-24 | E-GEOD-36405 | biostudies-arrayexpress

Histone H3 K36 trimethylation in wild-type BY4741 yeast strains

Project description:ChIP-on chip assays to measure the change in histone H3 K36 trimethylation over the yeast genome in wild-type yeast strains. Two color experiment.WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-37105 | biostudies-arrayexpress

Histone H3 K36me3 in wildtype, isw1 and chd1 yeast strains

Project description:ChIP-chip assays to measure the occupancy of histone H3 K36me3 over the yeast genome in wildtype, isw1 and chd1 yeast strains. ChIPs were done with K36me3 antibody (Ab 9050) in G1 arrested yeast cells. Two color experiment. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-32044 | biostudies-arrayexpress

Histone H3 K56 acetylation in wild-type histone shuffle strain (YBL574)

Project description:ChIP-on chip assays to measure the change in histone H3 K56 acetylation over the yeast genome in wild-type YBL574 yeast strains compared to H3K36A mutant strains. Two color experiment. Mutant vs WT cells. Biological replicates=3 per IP per cell type.

2012-07-24 | E-GEOD-37039 | biostudies-arrayexpress

ChIP-Seq of LET-418::3xFLAG in mixed stage early C.elegans embryos

Project description:Our aim was to identify the genome-wide direct LET-418 binding sites in early C.elegans embryos. a FLAG-tagged version of LET-418 was expressed in let-418(ts) mutants synchronised as early mixed stage embryos, and processed for ChIP followed by deep-sequencing. The obtained binding profile was compared to non-enriched input DNA and significant binding sites were identified.

2016-09-12 | E-MTAB-4716 | biostudies-arrayexpress

Genome-wide characterization of PhoB binding profile in Escherichia coli (ChIP-chip data)

Project description:Chromatin immunoprecipitation was combined with high-density tiling array (ChIP-chip) and gene expression microarray to reveal the adaptive responses of Escherichia coli to phosphate starvation. The first sketch of the genome-wide distribution of PhoB binding profile was unveiled and 43 regions were identified as the PhoB binding regions. The presence of a significant common motif in these binding regions allowed us to reconstruct the PhoB binding pattern. By comparing the ChIP-chip and microarray datasets, we were also able to identify genes directly or indirectly affected through PhoB regulation. Nineteen out of the 287 differentially expressed genes in the presence and absence of PhoB activity were considered as the genes directly regulated by PhoB. The adaptive responses affected through PhoB regulation are discussed and these responses involve in several important biological functions including transcriptional regulation, transportation, membrane component arrangement, sigma factor modulation and DNA replication inhibition. Comparison of the E. coli K12 MG1655 wild type and a PhoB-FLAG fusion expressing strain (MG1655_PhoB_FLAG). The anti-FLAG antibody was used to recognize PhoB-FLAG fusion protein. Therefore, ChIPed DNA from the wild type strain was used as the control group. Three biological replicates were performed.

2012-10-21 | E-GEOD-21856 | biostudies-arrayexpress

Genome-wide characterization of PhoB binding profile in Escherichia coli

Project description:This SuperSeries is composed of the following subset Series: GSE21820: Genome-wide characterization of PhoB binding profile in Escherichia coli (gene expression data) GSE21856: Genome-wide characterization of PhoB binding profile in Escherichia coli (ChIP-chip data) Refer to individual Series

2012-10-21 | E-GEOD-21857 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data