Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from Aspergillus niger comparing aerial structures with vegetative mycelium


ABSTRACT: A. niger undergoes dramatic changes during asexual development. We tried to identify the differences in RNA expression levels that are important for this development. We used micro-arrays to determine which genes were up- or down-regulated in the aerial structures, the part of the colony that is formed during asexual development. A. niger was grown as a sandwiched culture (Wösten et al., 1991,Journal of General Microbiology 137: 2017–2023) in a 0.25 mm layer of 0.6 % agarose between two porous polycarbonate membranes (diameter 76 mm, pore size 0.1 µm; Profiltra; www.profiltra.nl). After six days of growth, the top membrane of the sandwich was replaced by a membrane with pores of 10 µm (Profiltra), allowing formation of aerial hyphae and conidiophores for 24 h. Vegetative mycelium and aerial structures of 7-day-old maltose-grown cultures of A. niger were harvested from 3 and 5 sandwiched colonies, respectively. The aerial structures were scraped from the top membrane of the sandwiched culture with a razor blade. From other colonies, vegetative mycelium was harvested by flipping over the top membrane and scraping it off with a razor blade.

ORGANISM(S): Aspergillus niger

SUBMITTER: R Bleichrodt 

PROVIDER: E-GEOD-32123 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-02-24 | GSE32123 | GEO
2010-07-07 | E-GEOD-21752 | biostudies-arrayexpress
2010-07-07 | GSE21752 | GEO
2014-10-07 | E-GEOD-46187 | biostudies-arrayexpress
2014-03-13 | E-GEOD-51023 | biostudies-arrayexpress
2012-05-06 | E-GEOD-37795 | biostudies-arrayexpress
2011-01-31 | E-GEOD-21015 | biostudies-arrayexpress
2011-01-31 | E-GEOD-19952 | biostudies-arrayexpress
| PRJNA147445 | ENA
2014-08-22 | E-GEOD-57315 | biostudies-arrayexpress