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A549 expression profile induced by high levels of extracellular GPBP-1

ABSTRACT: Patients undergoing immune complex-mediated GN display increased levels of circulating GPBP-1 (cGPBP-1). To assess the relationship between high levels of cGPBP-1 and the onset of these diseases we incubated A549 cells with 200 ng/mL of recombinant extracellular GPBP-1 or with buffer (control). After 3 hours cells were lysed and total RNA extracted and assessed the mRNA expression. The functional analysis of data provide evidence that high levels of circulating GPBP-1 correlate with a lupus-prone cellular response. A549 cells were seeded in 6-well plates, grown for 18 hours and treated with 200 ng/mL of recombinant extracellular GPBP-1 or buffer (TBS). After three hours of incubation, cells were lysed, total RNA extracted and mRNA expression analysed following Agilent's recommendations.

ORGANISM(S): Homo sapiens

SUBMITTER: Fernando Revert

PROVIDER: E-GEOD-32181 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A549 expression profile induced by high levels of extracellular GPBP-1

Project description:Patients undergoing immune complex-mediated GN display increased levels of circulating GPBP-1 (cGPBP-1). To assess the relationship between high levels of cGPBP-1 and the onset of these diseases we incubated A549 cells with 200 ng/mL of recombinant extracellular GPBP-1 or with buffer (control). After 3 hours cells were lysed and total RNA extracted and assessed the mRNA expression. The functional analysis of data provide evidence that high levels of circulating GPBP-1 correlate with a lupus-prone cellular response.

2012-09-14 | GSE32181 | GEO

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Project description:A growing body of evidence suggests gene regulatory functions for the majority of non-protein-coding RNAs (ncRNAs). Besides small RNAs (sRNAs), the diverse class of long ncRNAs (lncRNAs) recently came into focus of research. So far, the relevance of lncRNAs in infection processes remains elusive. Here, we report the differential expression of several classes of lncRNAs during influenza A virus (IAV) infection in human lung epithelial cells. 2 biological replicates of each condition were hybridzed in an independent color-swap; A549 cells were washed with PBS and then infected with viruses at MOI 1 in infection buffer for for 60M-bM-^@M-^Imin at room temperature. Cells were incubated for the indicated time periods at 37M-bM-^@M-^IM-BM-0C in DMEM supplemented with 0.2% bovine serum albumin, 4M-bM-^@M-^ImM l-glutamine and antibiotics. Supernatants of A/WSN/33 (H1N1) virus infected A549 cells (MOI 5, 4hpi) were exposed to UV light for 5 min and then used to stimulate A549 cells for 4 h Supernatants of A/WSN/33 (H1N1) virus infected A549 cells (MOI 5, 8hpi) were exposed to UV light for 5 min and then used to stimulate A549 cells for 8 h

2014-01-29 | E-GEOD-45397 | biostudies-arrayexpress

The NS1 protein of influenza A virus suppresses interferon-regulated activation of antigen-presentation and immune-proteasome pathways

Project description:The NS1 protein of influenza virus counters host antiviral defences primarily by antagonizing the type I interferon (IFN) response. Both the N-terminal dsRNA-binding domain and the C-terminal effector domain are required for optimal suppression of host responses during infection. To better understand the regulatory role of the NS1 effector domain, we used an NS1-truncated mutant virus derived from human H1N1 influenza isolate A/Texas/36/91 (Tx/91) and assessed global transcriptional profiles from two independent human lung cell-culture models. Primary human tracheobronchial epithelial (HTBE) cells were washed and infected with 4×10^6 p.f.u. Tx/91 or Tx/91 NS1 : 1–126 viruses per filter (m.o.i. of 20) for 1 h at 37 °C. Three replicate wells were infected for each condition per time point. Allantoic fluid was used for mock treatments. At 9.5 and 25 h p.i., cells were collected and resuspended in TRIzol reagent (Invitrogen) for total RNA extraction. A549 cells were infected with Tx/91 or Tx/91 NS1 : 1–126 viruses at an m.o.i. of 2 for 1 h at 4 °C. Three replicate wells were infected for each condition per time point. Allantoic fluid was used for mock treatments. A549 cells were collected and lysed in solution D (4 M guanidinium thiocyanate, 25 mM sodium citrate, 0.5 % sarcosyl, 0.1 M β-mercaptoethanol) at 12 and 24 h p.i. for total RNA extraction.

2012-07-09 | E-GEOD-39200 | biostudies-arrayexpress

miRNA expression of lung cancer cells A549 and cisplatin (DDP) resistant lung cancer cells A549/DDP

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2016-08-16 | E-GEOD-85603 | biostudies-arrayexpress

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2017-12-18 | E-MTAB-5627 | biostudies-arrayexpress

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2011-04-01 | E-GEOD-28230 | biostudies-arrayexpress

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2012-07-09 | E-GEOD-39199 | biostudies-arrayexpress

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2012-05-13 | E-GEOD-37951 | biostudies-arrayexpress

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