Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Role of antigenic drive in pathogenesis of MALT lymphoma

ABSTRACT: In order to assess the effect that eradication therapy and re-infection have on colonization densities and histopathology of BALB/c mice, 35 mice were divided into four groups (A-D): 12 mice were infected for the duration of the experiment (23 months, primarily infected group B), 8 mice were infected together with group B, but also received a two week triple therapy at 18 months post infection (TT treated group C) and 9 additional mice were infected and treated along with group C and subsequently re-infected one month after the start of triple therapy (re-infected group D). The final group of 6 mice received no treatment or infection (control group A). All mice were sacrificed at 23 months post initial infection; their stomachs were removed and subjected to histopathological and gene expression analysis. The reference for all arrays used in this study consisted of pooled cDNA extracted from stomachs of five age-matched uninfected control animals. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract.

ORGANISM(S): Mus musculus

SUBMITTER: Anne Mueller

PROVIDER: E-GEOD-3237 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The role of antigenic drive and tumor-infiltrating accessory cells in the pathogenesis of helicobacter-induced mucosa-associated lymphoid tissue lymphoma.

Mueller Anne A O'rourke Jani J Chu Pauline P Chu Amanda A Dixon Michael F MF Bouley Donna M DM Lee Adrian A Falkow Stanley S

The American journal of pathology 20050901 3

Gastric B-cell lymphoma of mucosa-associated lymphoid tissue type is closely linked to chronic Helicobacter pylori infection. Most clinical and histopathological features of the tumor can be reproduced by prolonged Helicobacter infection of BALB/c mice. In this study, we have addressed the role of antigenic stimulation in the pathogenesis of the lymphoma by experimental infection with Helicobacter felis, followed by antibiotic eradication therapy and subsequent re-infection. Antimicrobial therap ...[more]

PMID: 16127158

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Project description:Summary: Breast cancer cell lines have been used widely to investigate breast cancer pathobiology and new therapies. Breast cancer is a molecularly heterogeneous disease, and it is important to understand how well and which cell lines best model that diversity. In particular, microarray studies have identified molecular subtypes (luminal A, luminal B, ERBB2-associated, basal-like and normal-like) with characteristic gene-expression patterns and underlying DNA copy number alterations (CNAs). Here, we studied a collection of breast cancer cell lines to catalog molecular profiles and to assess their relation to breast cancer subtypes. Whole-genome DNA microarrays were used to profile gene expression and CNAs in a collection of 52 widely-used breast cancer cell lines, and comparisons were made to existing profiles of primary breast tumors. Hierarchical clustering was used to identify gene-expression subtypes, and Gene Set Enrichment Analysis (GSEA) to discover biological features of those subtypes. Genomic and transcriptional profiles were integrated to discover within high-amplitude CNAs candidate cancer genes with coordinately altered gene copy number and expression. Transcriptional profiling of breast cancer cell lines identified one luminal and two basal-like (A and B) subtypes. Luminal lines displayed an estrogen receptor (ER) signature and resembled luminal-A/B tumors, basal-A lines were associated with ETS-pathway and BRCA1 signatures and resembled basal-like tumors, and basal-B lines displayed mesenchymal and stem-cell characteristics. Compared to tumors, cell lines exhibited similar patterns of CNA, but an overall higher complexity of CNA (genetically simple luminal-A tumors were not represented), and only partial conservation of subtype-specific CNAs. We identified 80 high-level DNA amplifications and 13 presumptive homozygous deletions, and the resident genes with concomitantly altered gene-expression, highlighting known and novel candidate breast cancer genes. Overall, breast cancer cell lines were genetically more complex than tumors, but retained expression patterns with relevance to the luminal-basal subtype distinction. The compendium of molecular profiles defines cell lines suitable for investigations of subtype-specific pathobiology, biomarkers and therapies, and provides a resource for discovery of new breast cancer genes. HEEBO oligonucleotide microarrays from the Stanford Functional Genomics Facility were used to perform gene expression profiling of 50 human breast epithelial cell lines, in comparison to a universal RNA reference. Expression data were analyzed by hierarchical clustering to identify subgroups, and gene set enrichment analysis to identify subgroup-specific gene pathways.

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Dataset Information

Role of antigenic drive in pathogenesis of MALT lymphoma

Publications

The role of antigenic drive and tumor-infiltrating accessory cells in the pathogenesis of helicobacter-induced mucosa-associated lymphoid tissue lymphoma.

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