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Transcription profiling of mouse Sca1-CD45 sorted bone marrow

ABSTRACT: Sca1-CD45 sorted bone marrow Experiment Overall Design: this experiment include 4 samples and 22 replicates

ORGANISM(S): Mus musculus

SUBMITTER: OGIC Info Ontario Genomics Innovation Centre (OGIC)

PROVIDER: E-GEOD-3241 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sca1-CD45 sorted bone marrow

Project description:Sca1-CD45 sorted bone marrow Keywords: other

2005-09-01 | GSE3241 | GEO

Gene expression profiling of CD45+/Sca1+ cells isolated from the bone marrow and the muscle

Project description:The BM-derived CD45+/Sca1+ cells are haematopoietic stem/progenitor cells that have the ability to circulate and migrate and engraft to the muscle tissue, and therefore they are of particular interest. Notably, these cells retain their haematopoietic potential, as revealed both by in vitro and in vivo assays; but they also acquire myogenic potential, as shown by their ability to participate in muscle regeneration. Whether, this latter remarkable ability is the result of the reprogramming of the BM-CD45+/Sca1+ cells and the activation of a myogenic molecular program within these cells, remains controversial. This study aims to clarify this aspect of the process, investigating the role of the muscle microenviroment and key myogenic transcription factors. Keywords: CD45+/Sca1+ cells, BM, muscle CD45+/Sca1+ cells isolated from the BM or the muscle were processed fresh and their RNA was extracted. Moreover, CD45+/Sca1+ cells isolated from the muscle of BM transplanted or untransplanted mice after injury with Cardiotoxin were processed fresh and their RNA was extracted.

2010-04-05 | E-GEOD-9296 | biostudies-arrayexpress

Gene expression profiling of CD45+/Sca1+ cells isolated from the bone marrow and the muscle

2010-04-06 | GSE9296 | GEO

Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

Project description:Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

2024-01-23 | GSE216080 | GEO

Transcription profiling of mouse putative muscle derived stem cells

Project description:Comparison of NRA derived from SCA1+, CD45- MACS purified putative muscle derived stem cells vs. RNA derived from preplating of hind limb muscle cells. Experiment Overall Design: this experiment include 2 samples and 8 replicates

2008-06-12 | E-GEOD-3773 | biostudies-arrayexpress

CD45+ vs CD45- RCC proteome characterization

Project description:Exploration of proteome differences between CD45+ and CD45- cell types in renal cell carcinoma tumors and normal adjacent tissue patient samples.

2020-10-14 | PXD014370 | Pride

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [scRNA-Seq]

Project description:Although hematopoietic stem and progenitor cells (HSPCs) become activated in the cell-cycle status after chemotherapy to supply hematopoietic loss, the detailed mechanisms of activation remain unknown. Here we show that Sca1+ macrophages play a key role for bone marrow (BM) recovery through granulocyte-macrophage colony-stimulating factor (GM-CSF) secretion. By analyzing gene expression profiles of HSPCs lodged in 5-fluolouracil (5-FU)-treated mice, we found GM-CSF as a key proliferative signal. Sca1+ macrophages in BM after 5-FU treatment expressed high levels of GM-CSF. GM-CSF-knockout mice treated with 5-FU were lethal because of severe BM suppression. Up-regulation of Csf2 in Sca1+ macrophages by 5-FU was suppressed in MyD88-knockout mice, suggesting that TLR signaling via damage-associated molecular patterns caused by cell death is critical for up-regulation of Csf2. In 5-FU treated BM, majority of Sca1+ macrophages and transplanted HSPCs locate perivascular areas. These findings together indicate that Sca1+ macrophages induce HSPCs to proliferate through GM-CSF signaling in the stressed BM environments.

2021-01-20 | GSE120112 | GEO

Sca1+ macrophages activate hematopoietic stem/progenitor cells upon bone marrow stress. [RNA-Seq]

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Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

Project description:Expression profile comparing of sorted 4T1-Sca1+ and 4T1-Sca1- cells

| PRJNA892045 | ENA

Transcription profiling of mouse Sca1+ primary myospheres isolated from Balb/c animals

Project description:Cells were isolated from muscle (hind leg) of Balb/c mouse (male). Then cells were enriched at FACS by using Sca1 antigen. Cells were grown 4 days in NSC medium, passaged and RNA isolated. Cells were at passage 1. Experiment Overall Design: this experiment include 1 samples and 6 replicates

2008-06-12 | E-GEOD-3235 | biostudies-arrayexpress

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