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BMM_BalbC_LPS_timecourse

ABSTRACT: Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity

ORGANISM(S): Mus musculus

SUBMITTER: David Hume

PROVIDER: E-GEOD-325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Continued discovery of transcriptional units expressed in cells of the mouse mononuclear phagocyte lineage.

Wells Christine A CA Ravasi Timothy T Sultana Razvan R Yagi Ken K Carninci Piero P Bono Hidemasa H Faulkner Geoffrey G Okazaki Yasushi Y Quackenbush John J Hume David A DA Lyons Paul A PA

Genome research 20030601 6B

The current RIKEN transcript set represents a significant proportion of the mouse transcriptome but transcripts expressed in the innate and acquired immune systems are poorly represented. In the present study we have assessed the complexity of the transcriptome expressed in mouse macrophages before and after treatment with lipopolysaccharide, a global regulator of macrophage gene expression, using existing RIKEN 19K arrays. By comparison to array profiles of other cells and tissues, we identify ...[more]

PMID: 12819134

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BMM_C3H/ARC_LPS_timecourse

Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity

2010-06-05 | E-GEOD-324 | biostudies-arrayexpress

BMM_C3H/HeJ_LPS_timecourse

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2010-06-05 | E-GEOD-6435 | biostudies-arrayexpress

Macrophage response to lipopolysaccharide

Project description:Temporal analysis of bone marrow derived macrophages after 10 ug/ml lipopolysaccharide stimulation. C57BL6, C3H/ARC, BalbC and C3H/HeJ mouse strains analyzed. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5.

2010-07-01 | E-GEOD-3148 | biostudies-arrayexpress

Clontech RNA samples

Project description:A series of experiments used to compare Clontech spleen plyA, Clontech liver plyA and Clontech universal RNA for microarrays on our available human array printed on Amersham type 7 * slides. Keywords = human, clontech, automated slide processor, spleen, liver, universal RNA

2010-05-27 | E-GEOD-40 | biostudies-arrayexpress

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Project description:Hybridization of one kidney of cortisol treated fish vs. one kidney of control fish. Kidneys were collected from untreated juvenile sea bream (n=4) and from fish, which received for 72h a coconut-oil implant containing 10mg/Kg (fish wet weight) (n=4) cortisol. Experiments were carried out at the University of the Algarve, Portugal in accordance with National legislation for the welfare of animals. Experiments were conducted in two 125 l cylindriconical tanks supplied with a continuous through-flow of oxygenated seawater at 20+1 Â°C using juvenile sea bream (25 g + 3 g) adapted for 1 week to the experimental conditions. One tank contained 8 untreated fish (control) and the other tank 8 cortisol treated fish and the end of experiments fish were removed form tanks, decapitated and the kidneys rapidly removed and place in RNAlater (Qiagen) at â20 Â°C. No mortality occurred in the control tank but 2 fish died in the cortisol treated tank.

2010-06-11 | E-GEOD-2064 | biostudies-arrayexpress

Tiling-set aCGH data for oral dysplasias and oral squamous cell carcinomas

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2008-06-15 | E-GEOD-7472 | biostudies-arrayexpress

Transcription profiling of rat colon after dietary treatment with fructo oligosaccharides

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2008-06-14 | E-GEOD-5943 | biostudies-arrayexpress

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