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CD4 on human monocytes

ABSTRACT: We examined the effects of ligating CD4 expressed by primary human peripheral blood monocytes with soluble MHC Class II. 3 replicates are included. Fresh blood monocytes, obtained through negative selection via magnetic activated cell sorting (Miltenyi, Auburn, CA), from three separate individual donors were treated with RPMI-1640 medium alone or with medium containing sMHC II (1 ug/ml) for three hours. Cells were harvested and RNA purified using the RNeasy procedure (Qiagen). RNA was then analyzed by the UCLA Clinical Microarray Core Laboratory using the Human U133 Plus 2.0 Array (Affimetrix). Data was analyzed using Dchip software. Visual inspection did not find any potential outliers or suspect areas. Data across all groups were normalized using the PM/MM difference.

ORGANISM(S): Homo sapiens

SUBMITTER: Scott Kitchen

PROVIDER: E-GEOD-32939 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection.

Zhen Anjie A Krutzik Stephan R SR Levin Bernard R BR Kasparian Saro S Zack Jerome A JA Kitchen Scott G SG

Journal of virology 20140618 17

<h4>Unlabelled</h4>A unique aspect of human monocytes, compared to monocytes from many other species, is that they express the CD4 molecule. However, the role of the CD4 molecule in human monocyte development and function is not known. We determined that the activation of CD4 via interaction with major histocompatibility complex class II (MHC-II) triggers cytokine expression and the differentiation of human monocytes into functional mature macrophages. Importantly, we determined that CD4 activat ...[more]

PMID: 24942581

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