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Transcription profiling of rat proximal femoral growthplate after mid-shaft fracture

ABSTRACT: Mid-shaft fracture stimulates bone lengthening by increasing linear growth at the growthplate. This project studied changes in mRNA in the proximal growthplate after a mid-shaft fracture in a rat model. Experiment Overall Design: Study of rat proximal femoral growthplate after mid-shaft fracture in 4 week old Sprague-Dawley female rats. RNA from two rats were pooled for each sample. Proximal femoral growthplate was studied from the fractured femora and the intact contralateral femora.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ralph Meyer

PROVIDER: E-GEOD-3298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Evidence for overgrowth after midfemoral fracture via increased RNA for mitosis.

Ashraf Nomaan N Meyer Martha H MH Frick Steven S Meyer Ralph A RA

Clinical orthopaedics and related research 20070101

Middiaphyseal femoral fractures in children and young rats stimulate linear femoral growth, a phenomenon commonly attributed to increased vascularity. To test for changes in mRNA expression of genes related to blood vessels, nerve fibers, cartilage, bone, and cell metabolism, we measured mRNA gene expression for all known rat genes in the physis at various times after diaphyseal fracture. Female Sprague-Dawley rats, 4 weeks of age at surgery, were subjected to a unilateral, simple, transverse, m ...[more]

PMID: 16924177

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