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Activated YAP1 stimulates cardiomyocyte proliferation

ABSTRACT: Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later. 2 group comparison, n=4 biological replicates per group

ORGANISM(S): Rattus norvegicus

SUBMITTER: William Pu

PROVIDER: E-GEOD-33019 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Activated YAP1 stimulates cardiomyocyte proliferation

Project description:Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later.

2012-01-12 | GSE33019 | GEO

Regulation of gene expression by YAP1

Project description:In order to define YAP1-specific gene expression patterns in gastric cancer, the constitutively active mutant YAP1 (YAP1-S127A) was over-expressed in MKN45 gastric cancer cells. Defined gene expression signature was later used to stratify gastric cancer patients according to the presence of the YAP1-activated signature. Three groups of samples are included: 1. Mock control; 2. Vector control; 3. YAP-S127A expression. Gene expression profiles of YAP-S127A mutant-expressing cells were compared to that of mock and vector control. Experiments were done in MKN45 gastric cancer cells.

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Regulation of gene expression by YAP1

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RASSF1A promotes its tumor-suppressive function via YAP1 target genes

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AP-MS_XL experiment to characterize YAP1/LATS/PTPN14 complex

Project description:In this project we investigated the topological composition of ternary complex PTPN14/LATS1/PTPN14. For this purpose, we performed affinity purification with Strep tag of Strep-HA YAP1 and subsequent XL reaction.

2023-03-04 | PXD039440 | Pride

Role of Yes-Associated Protein 1 (YAP1) in rhabdomyosarcoma

Project description:Doxycycline-inducible YAP1 S127A-driven rhabdomyosarcoma (RMS) tumors, control skeletal muscle and regressed tumors following YAP1 normalization by doxycycline withdrawal were compared to determine the YAP1-regulated gene expression profile relevant to RMS formation. To characterize the role of YAP1 in embryonal RMS at the molecular level and identify a gene signature for YAP1 activity readout, we compared the gene expression profiles of our YAP1-driven ERMS with control donor skeletal muscle (SKM) and doxycycline-withdrawn regressing tumors by microarray (doxycycline withdrawal for 3 or 6 days; OFF3 and OFF6, respectively). We next extracted a list of genes regulated by YAP1 in our YAP1-driven ERMS tumors (TUM) versus the 3 other conditions: skeletal muscle control (SKM), Doxycycline-withdrawn 3 days (OFF3) and 6 days (OFF6). The overlap between the 3 lists identified a subset of 633 common upregulated genes, named the YAP1-ERMS_UP signature, as well as 249 common downregulated genes, termed the YAP1-ERMS_DOWN signature. Proliferative pathways and transcriptional targets of E2F factors were highlighted in the YAP1-ERMS_UP genes, while muscle differentiation and trancriptional targets of myogenic factors Myod1 and Mef2 were highlighted in the YAP1-ERMS_DOWN genes. Tumor regression conditions (OFF3 days; OFF6 days) as well as control muscle (CTL) were compared with tumors at day 0 of doxycycline withdrawal (TUM). 3 samples for each conditions were used.

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AP-MS of Strep-HA YAP1 mutants (S61A, S109A, S127A, S138A, S143A, S367A, 5SA)

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human hTERT-RPE1 cell spheroid : Control siRNA transfected vs. YAP1 siRNA transfected

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