Comparison of the transcriptome of day 3 human embryo with that of the trophectoderm
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Said Assou
PROVIDER: E-GEOD-33025 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Transcriptome analysis during human trophectoderm specification suggests new roles of metabolic and epigenetic genes.
Assou Said S Boumela Imène I Haouzi Delphine D Monzo Cécile C Dechaud Hervé H Kadoch Issac-Jacques IJ Hamamah Samir S
PloS one 20120622 6
In humans, successful pregnancy depends on a cascade of dynamic events during early embryonic development. Unfortunately, molecular data on these critical events is scarce. To improve our understanding of the molecular mechanisms that govern the specification/development of the trophoblast cell lineage, the transcriptome of human trophectoderm (TE) cells from day 5 blastocysts was compared to that of single day 3 embryos from our in vitro fertilization program by using Human Genome U133 Plus 2.0 ...[more]
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Project description:In humans, the embryonic genome activation (EGA) program is functional by day 3 after fertilization. The 6-8 cell stage embryo (day 3 post-fertilization) starts the process of “compaction” that leads to the generation of the tightly organized cell mass of the morula and is followed by differentiation of the morula into a blastocyst. The transition from day 3 embryos to day 5 blastocysts is likely to be controlled by many and specific changes in the expression of different genes. We used mRNA amplification technique and compared the transcriptomes of day 3 human embryos and trophectoderm (TE) cells from day 5 human blastocysts to identify transcripts that are differentially expressed during the embryo-to-TE transition and involved in the TE specification.
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